| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARL16 |
| Uniprot No | Q0P5N6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-197aa |
| 氨基酸序列 | MRVAGGRALS RGAELRVPGG AKHGMCLLLG ATGVGKTLLV KRLQEVSSRD GKGDLGEPPP TRPTVGTNLT DIVAQRKITI RELGGCMGPI WSSYYGNCRS LLFVMDASDP TQLSASCVQL LGLLSAEQLA EASVLILFNK IDLPCYMSTE EMKSLIRLPD IIACAKQNIT TAEISAREGT GLAGVLAWLQ ATHRAND |
| 分子量 | 20.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人腺苷二磷酸核糖基化因子样蛋白16(ARL16)的模拟参考文献示例(注：内容为虚构示例，非真实文献)：
1. **文献名称**：**"ARL16 regulates mitochondrial dynamics through interaction with fission proteins"**
**作者**：Smith A et al. (2005)
**摘要**：首次克隆并表征ARL16的结构，发现其广泛表达于肝脏、脑组织及免疫细胞中。研究证明ARL16通过结合线粒体分裂蛋白(如DRP1)，调控线粒体形态与功能，影响细胞能量代谢。
2. **文献名称**：**"ARL16 modulates innate immune responses via TLR/NF-κB signaling"**
**作者**：Jones B et al. (2012)
**摘要**：探讨ARL16在巨噬细胞中的免疫功能，发现其通过抑制TLR4介导的NF-κB通路，负调控炎症因子(如TNF-α、IL-6)的产生，可能成为炎症性疾病的潜在治疗靶点。
3. **文献名称**：**"Downregulation of ARL16 in hepatocellular carcinoma promotes tumor proliferation"**
**作者**：Zhang C et al. (2017)
**摘要**：分析肝癌组织中ARL16的表达显著降低，体外实验显示过表达ARL16可抑制癌细胞增殖并诱导凋亡，机制与阻断MAPK通路活化相关，提示其作为肿瘤抑制因子的潜力。
4. **文献名称**：**"Structural basis of ARL16 GTPase activity in vesicle trafficking"**
**作者**：Brown D et al. (2020)
**摘要**：通过X射线晶体学解析ARL16的GTP结合结构域，阐明其GTP水解机制，并发现ARL16与细胞内转运蛋白(如COPI/II复合物)互作，推测其参与高尔基体到内质网的膜泡运输。
(以上示例为学术研究常见方向的模拟，实际文献需通过数据库如PubMed检索获取。)
Recombinant human ADP-ribosylation factor-like protein 16 (ARL16) is a member of the ARL family within the Ras superfamily of small GTPases. These proteins are structurally homologous to ADP-ribosylation factors (ARFs) but lack detectable ADP-ribosylation activity. ARL16 is encoded by the *ARL16* gene and is evolutionarily conserved across eukaryotes, suggesting fundamental cellular roles. While its precise biological functions remain under investigation, ARL16 is implicated in intracellular trafficking, membrane dynamics, and signal transduction pathways common to GTPase regulatory systems. Unlike some ARL proteins with well-characterized roles (e.g., ARL1 in Golgi-vesicle transport), ARL16's specific molecular interactions and effectors are less defined. Preliminary studies link it to immune modulation, as ARL16 expression is upregulated in certain autoimmune conditions, potentially influencing cytokine signaling or antigen presentation. Its recombinant form, generated via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables structural and functional studies, including GTP-binding assays, interactome mapping, and knockout/rescue experiments. Recent advances in cryo-EM and protein-protein interaction screening have begun to clarify its tertiary structure and binding partners, though mechanistic insights into its regulatory network remain sparse. ARL16's recombinant production also holds translational potential for developing biomarker assays or targeted therapies, particularly in autoimmune or inflammatory disorders. Further research is required to elucidate its subcellular localization, tissue-specific expression patterns, and disease-associated variants.
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