| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARID5B |
| Uniprot No | Q14865 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1188aa |
| 氨基酸序列 | MEPNSLQWVGSPCGLHGPYIFYKAFQFHLEGKPRILSLGDFFFVRCTPKDPICIAELQLLWEERTSRQLLSSSKLYFLPEDTPQGRNSDHGEDEVIAVSEKVIVKLEDLVKWVHSDFSKWRCGFHAGPVKTEALGRNGQKEALLKYRQSTLNSGLNFKDVLKEKADLGEDEEETNVIVLSYPQYCRYRSMLKRIQDKPSSILTDQFALALGGIAVVSRNPQILYCRDTFDHPTLIENESICDEFAPNLKGRPRKKKPCPQRRDSFSGVKDSNNNSDGKAVAKVKCEARSALTKPKNNHNCKKVSNEEKPKVAIGEECRADEQAFLVALYKYMKERKTPIERIPYLGFKQINLWTMFQAAQKLGGYETITARRQWKHIYDELGGNPGSTSAATCTRRHYERLILPYERFIKGEEDKPLPPIKPRKQENSSQENENKTKVSGTKRIKHEIPKSKKEKENAPKPQDAAEVSSEQEKEQETLISQKSIPEPLPAADMKKKIEGYQEFSAKPLASRVDPEKDNETDQGSNSEKVAEEAGEKGPTPPLPSAPLAPEKDSALVPGASKQPLTSPSALVDSKQESKLCCFTESPESEPQEASFPSFPTTQPPLANQNETEDDKLPAMADYIANCTVKVDQLGSDDIHNALKQTPKVLVVQSFDMFKDKDLTGPMNENHGLNYTPLLYSRGNPGIMSPLAKKKLLSQVSGASLSSSYPYGSPPPLISKKKLIARDDLCSSLSQTHHGQSTDHMAVSRPSVIQHVQSFRSKPSEERKTINDIFKHEKLSRSDPHRCSFSKHHLNPLADSYVLKQEIQEGKDKLLEKRALPHSHMPSFLADFYSSPHLHSLYRHTEHHLHNEQTSKYPSRDMYRESENSSFPSHRHQEKLHVNYLTSLHLQDKKSAAAEAPTDDQPTDLSLPKNPHKPTGKVLGLAHSTTGPQESKGISQFQVLGSQSRDCHPKACRVSPMTMSGPKKYPESLSRSGKPHHVRLENFRKMEGMVHPILHRKMSPQNIGAARPIKRSLEDLDLVIAGKKARAVSPLDPSKEVSGKEKASEQESEGSKAAHGGHSGGGSEGHKLPLSSPIFPGLYSGSLCNSGLNSRLPAGYSHSLQYLKNQTVLSPLMQPLAFHSLVMQRGIFTSPTNSQQLYRHLAAATPVGSSYGDLLHNSIYPLAAINPQAAFPSSQLSSVHPSTKL |
| 分子量 | 132 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARID5B的模拟参考文献示例(非真实文献，仅供格式参考)：
1. **标题**：ARID5B调控急性淋巴细胞白血病中糖皮质激素反应的分子机制
**作者**：Smith J, et al.
**摘要**：研究发现ARID5B基因的遗传变异通过影响白血病细胞对糖皮质激素的敏感性，与儿童ALL的化疗耐药性相关，揭示其作为转录因子在药物反应通路中的调控作用。
2. **标题**：ARID5B在脂肪细胞分化中的表观遗传调控功能
**作者**：Li X, Wang Y.
**摘要**：通过CRISPR筛选发现，ARID5B通过结合脂肪生成相关基因启动子的AT富集区域，招募组蛋白修饰复合物，促进成脂分化的表观遗传重编程。
3. **标题**：ARID5B基因多态性与自身免疫疾病易感性的全基因组关联研究
**作者**：Garcia R, et al.
**摘要**：大规模GWAS分析证实ARID5B rs10821944位点多态性与类风湿性关节炎和系统性红斑狼疮的易感性显著相关，可能通过调控免疫细胞中IL-6等炎性因子的表达。
4. **标题**：ARID5B介导的染色质重塑在胚胎发育中的动态作用
**作者**：Chen T, et al.
**摘要**：利用斑马鱼模型证明，ARID5B通过动态调控中胚层分化相关基因的染色质可及性，影响早期胚胎组织发育，缺失导致心脏畸形及造血缺陷。
注：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar等平台检索确认。
ARID5B (AT-rich interaction domain 5B) is a member of the ARID family of DNA-binding proteins, characterized by a conserved ARID domain that preferentially interacts with AT-rich sequences in gene regulatory regions. It functions as a transcription regulator involved in chromatin remodeling, embryonic development, cell differentiation, and immune response modulation. Structurally, ARID5B contains an N-terminal ARID domain essential for DNA binding and a C-terminal region mediating protein-protein interactions.
Studies link ARID5B to both physiological and pathological processes. It regulates adipogenesis, hematopoiesis, and neural development, while also influencing inflammatory pathways by modulating cytokine production (e.g., IL-6. TNF-α). Notably, single-nucleotide polymorphisms (SNPs) in the ARID5B gene are strongly associated with susceptibility to autoimmune diseases (e.g., rheumatoid arthritis, systemic lupus erythematosus) and childhood acute lymphoblastic leukemia (ALL). In cancer, ARID5B overexpression correlates with chemotherapy resistance and poor prognosis in ALL, possibly through dysregulation of cell cycle and apoptosis-related genes.
As a recombinant protein, ARID5B is utilized to study its interactions with transcriptional complexes like SWI/SNF and its role in epigenetic regulation. Current research focuses on its potential as a therapeutic target and biomarker for autoimmune disorders and malignancies.
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