| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARL5 |
| Uniprot No | Q9Y689 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-179aa |
| 氨基酸序列 | MGILFTRIWRLFNHQEHKVIIVGLDNAGKTTILYQFSMNEVVHTSPTIGSNVEEIVINNTRFLMWDIGGQESLRSSWNTYYTNTEFVIVVVDSTDRERISVTREELYKMLAHEDLRKAGLLIFANKQDVKECMTVAEISQFLKLTSIKDHQWHIQACCALTGEGLCQGLEWMMSRLKIR |
| 分子量 | 45.43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ARL5蛋白的3篇参考文献示例(注：文献为示例性内容，实际研究中请根据具体需求检索最新论文)：
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1. **文献名称**：*ARL5 regulates mitochondrial trafficking and homeostasis in polarized cells*
**作者**：Chen et al.
**摘要**：研究揭示了ARL5蛋白在细胞线粒体动态分布中的作用，通过重组ARL5蛋白体外表达，证明其通过结合GTP调控驱动蛋白互作，影响线粒体在细胞内的定向运输。
2. **文献名称**：*Expression, purification, and structural analysis of recombinant human ARL5 for functional studies*
**作者**：Zhang et al.
**摘要**：报道了利用大肠杆菌系统高效表达和纯化重组ARL5蛋白的方法，并通过X射线晶体学解析了其三维结构，为ARL5的GTP酶活性机制提供了结构基础。
3. **文献名称**：*ARL5 interacts with the retromer complex to modulate Wnt signaling pathways*
**作者**：Lee et al.
**摘要**：通过重组ARL5蛋白的体外结合实验及细胞模型，证明ARL5与逆向运输复合体(retromer)相互作用，参与调控Wnt信号通路的囊泡运输过程。
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**建议**：实际文献可通过PubMed或Google Scholar搜索关键词“ARL5 recombinant”“ARL5 GTPase”“ARL5 trafficking”获取最新研究。
**Background of Recombinant Human ARL5 Protein**
ARL5 (ADP-ribosylation factor-like protein 5) belongs to the ARF/ARL family of small GTP-binding proteins, which regulate membrane trafficking, cytoskeletal dynamics, and signaling pathways. ARL5 shares structural homology with other ARL members, featuring conserved GTP-binding domains that enable its switching between active (GTP-bound) and inactive (GDP-bound) states. Although less characterized than other ARL proteins, ARL5 is implicated in intracellular transport, lipid metabolism, and cell proliferation. Studies suggest its localization to the Golgi apparatus and endosomes, where it may modulate vesicle formation and cargo sorting.
ARL5 is encoded by the *ARL5A* and *ARL5B* paralogs in humans, with ARL5A being more extensively studied. Emerging research links ARL5A to cancer progression, particularly through interactions with oncogenic pathways like Wnt/β-catenin. Dysregulation of ARL5 expression has been observed in colorectal and gastric cancers, hinting at potential roles in tumorigenesis. Additionally, ARL5B is associated with metabolic disorders, including obesity and insulin resistance, possibly via lipid droplet regulation.
Recombinant ARL5 protein, produced using bacterial or mammalian expression systems, serves as a critical tool for functional studies, structural analysis (e.g., GTPase activity assays), and therapeutic target exploration. Its recombinant form enables precise investigation into molecular mechanisms and interactions, advancing insights into ARL5's physiological and pathological roles. Further research is needed to fully elucidate its biological significance and translational potential. (Word count: 249)
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