| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARNTL2 |
| Uniprot No | Q8WYA1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-636aa |
| 氨基酸序列 | MAAEEEAAAG GKVLREENQC IAPVVSSRVS PGTRPTAMGS FSSHMTEFPR KRKGSDSDPS QSGIMTEKVV EKLSQNPLTY LLSTRIEISA SSGSRVEDGE HQVKMKAFRE AHSQTEKRRR DKMNNLIEEL SAMIPQCNPM ARKLDKLTVL RMAVQHLRSL KGLTNSYVGS NYRPSFLQDN ELRHLILKTA EGFLFVVGCE RGKILFVSKS VSKILNYDQA SLTGQSLFDF LHPKDVAKVK EQLSSFDISP REKLIDAKTG LQVHSNLHAG RTRVYSGSRR SFFCRIKSCK ISVKEEHGCL PNSKKKEHRK FYTIHCTGYL RSWPPNIVGM EEERNSKKDN SNFTCLVAIG RLQPYIVPQN SGEINVKPTE FITRFAVNGK FVYVDQRATA ILGYLPQELL GTSCYEYFHQ DDHNNLTDKH KAVLQSKEKI LTDSYKFRAK DGSFVTLKSQ WFSFTNPWTK ELEYIVSVNT LVLGHSEPGE ASFLPCSSQS SEESSRQSCM SVPGMSTGTV LGAGSIGTDI ANEILDLQRL QSSSYLDDSS PTGLMKDTHT VNCRSMSNKE LFPPSPSEMG ELEATRQNQS TVAVHSHEPL LSDGAQLDFD ALCDNDDTAM AAFMNYLEAE GGLGDPGDFS DIQWTL |
| 分子量 | 70 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ARNTL2的核心文献简介(人工总结示例，非真实文献)：
1. **"ARNTL2 regulates circadian rhythm and tumor progression in colorectal cancer"**
- **作者**: Lee, S. et al.
- **摘要**: 发现ARNTL2通过与CLOCK蛋白形成异源二聚体调控肠道上皮细胞昼夜节律基因表达，其表达缺失可加速结直肠癌小鼠模型的肿瘤生长，提示其抑癌功能。
2. **"BMAL2 interacts with HIF-1α to mediate hypoxia-induced metabolic reprogramming"**
- **作者**: Wang, Y. et al.
- **摘要**: 在缺氧条件下，ARNTL2与HIF-1α协同激活糖酵解相关基因，促进乳腺癌细胞能量代谢适应，可能成为肿瘤治疗的潜在靶点。
3. **"Developmental expression of ARNTL2 in mouse embryos"**
- **作者**: Kobayashi, T. et al.
- **摘要**: 通过基因敲除模型发现，ARNTL2在小鼠胚胎心脏和神经发育中发挥关键作用，其缺失导致胚胎期致死及心脏发育缺陷。
注：以上文献名称及内容为虚构示例，实际研究中建议通过PubMed或Google Scholar检索真实文献(关键词：ARNTL2/BMAL2. circadian rhythm, cancer)。
ARNTL2 (Aryl Hydrocarbon Receptor Nuclear Translocator-Like Protein 2), also known as BMAL2. is a member of the basic helix-loop-helix PER-ARNT-SIM (bHLH-PAS) transcription factor family. It shares structural homology with BMAL1 (ARNTL), a core component of the mammalian circadian clock machinery. Both proteins contain conserved domains critical for dimerization and DNA binding, particularly the bHLH and PAS domains. While BMAL1 is widely studied for its role in circadian rhythm regulation, ARNTL2 exhibits overlapping but distinct functions. It is expressed in various tissues, including the brain, kidney, and lungs, often co-expressed with BMAL1 but showing tissue-specific variation in abundance.
ARNTL2 interacts with circadian regulators like CLOCK or NPAS2 to form heterodimers, binding E-box elements in promoters of target genes such as Period (Per) and Cryptochrome (Cry), thereby influencing circadian transcriptional cycles. However, its circadian role appears less dominant than BMAL1. suggesting functional redundancy or context-dependent specialization. Emerging evidence links ARNTL2 to non-circadian processes, including cellular metabolism, differentiation, and tumorigenesis. Dysregulation of ARNTL2 has been implicated in cancer progression, metabolic disorders, and neurodevelopmental conditions, though mechanistic insights remain limited. Genetic studies associate ARNTL2 polymorphisms with disease susceptibility, yet its precise pathophysiological roles and regulatory networks are still under investigation, making it a promising target for exploring molecular interactions between circadian biology and disease pathways.
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