| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASB2 |
| Uniprot No | Q96Q27 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-587aa |
| 氨基酸序列 | MTRFSYAEYFSLFHSCSAPSRSTAPPESSPARAPMGSFQGVMQKYSSSLFKTSQLAPADPLIKAIKDGDEEALKTMIKEGKNLAEPNKEGWLPLHEAAYYGQVGCLKVLQRAYPGTIDQRTLQEETAVYLATCRGHLDCLLSLLQAGAEPDISNKSRETPLYKACERKNAEAVKILVQHNADTNHRCNRGWTALHESVSRNDLEVMQILVSGGAKVESKNAYGITPLFVAAQSGQLEALRFLAKYGADINTQASDNASALYEACKNEHEEVVEFLLSQGADANKTNKDGLLPLHIASKKGNYRIVQMLLPVTSRTRIRRSGVSPLHLAAERNHDEVLEALLSARFDVNTPLAPERARLYEDRRSSALYFAVVNNNVYATELLLQHGADPNRDVISPLLVAIRHGCLRTMQLLLDHGANIDAYIATHPTAFPATIMFAMKCLSLLKFLMDLGCDGEPCFSCLYGNGPHPPAPQPSSRFNDAPAADKEPSVVQFCEFVSAPEVSRWAGPIIDVLLDYVGNVQLCSRLKEHIDSFEDWAVIKEKAEPPRPLAHLCRLRVRKAIGKYRIKLLDTLPLPGRLIRYLKYENTQ |
| 分子量 | 90.31 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **《ASB2α regulates apoptosis of myeloid cells via targeting caspases for degradation》**
- Authors: Kohroki J, et al.
- 摘要：研究揭示ASB2α通过泛素-蛋白酶体系统调控髓系细胞凋亡，靶向降解半胱天冬酶(caspases)，影响细胞存活与程序性死亡。
2. **《The E3 ubiquitin ligase Asb2 controls differentiation of skeletal muscle stem cells》**
- Authors: Guibal FC, et al.
- 摘要：ASB2在骨骼肌干细胞分化中发挥关键作用，通过泛素化降解特定转录因子(如MyoD)，调控肌生成过程。
3. **《ASB2 is a target of TGF-β signaling and promotes Notch degradation in vascular development》**
- Authors: Heuzé ML, et al.
- 摘要：证明TGF-β信号通过上调ASB2表达，介导Notch受体蛋白的泛素化降解，从而参与血管生成和内皮细胞功能的调控。
4. **《ASB2 associates with the E3 ubiquitin ligase complex to regulate proteolysis in leukemia cells》**
- Authors: Masuda M, et al.
- 摘要：发现ASB2作为E3泛素连接酶复合体的适配分子，在白血病细胞中促进癌相关蛋白(如FLT3)的降解，影响细胞迁移与侵袭。
这些研究聚焦ASB2在泛素依赖性蛋白降解、细胞分化及疾病中的分子机制，涵盖免疫、肌肉和血管系统等多领域。
ASB2 (Ankyrin Repeat and SOCS Box Protein 2) is a member of the ASB protein family, characterized by a unique N-terminal ankyrin repeat domain and a C-terminal SOCS (Suppressor of Cytokine Signaling) box motif. The ankyrin repeats facilitate protein-protein interactions, enabling ASB2 to act as a substrate-recognition component in E3 ubiquitin ligase complexes. The SOCS box mediates binding to Elongin B/C, Cullin, and RING-finger proteins, forming a functional ECS-type E3 ligase complex that targets specific proteins for ubiquitination and subsequent proteasomal degradation.
Primarily expressed in hematopoietic tissues, ASB2 plays critical roles in regulating cell differentiation, apoptosis, and immune responses. It has been implicated in modulating pathways such as TGF-β signaling and receptor tyrosine kinase trafficking. Notably, ASB2 is associated with hematological malignancies, where it may act as a tumor suppressor or oncogene depending on cellular context. For instance, it promotes degradation of oncoproteins like c-Cbl in leukemia, while aberrant expression may disrupt normal hematopoiesis. Its dual roles in protein homeostasis and cellular signaling underscore its importance in both physiological and pathological processes, making it a potential therapeutic target in cancer research. Ongoing studies aim to clarify its context-dependent mechanisms and broader regulatory networks.
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