| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASAM |
| Uniprot No | Q9H6B4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-373aa |
| 氨基酸序列 | MSLLLLLLLVSYYVGTLGTHTEIKRVAEEKVTLPCHHQLGLPEKDTLDIEWLLTDNEGNQKVVITYSSRHVYNNLTEEQKGRVAFASNFLAGDASLQIEPLKPSDEGRYTCKVKNSGRYVWSHVILKVLVRPSKPKCELEGELTEGSDLTLQCESSSGTEPIVYYWQRIREKEGEDERLPPKSRIDYNHPGRVLLQNLTMSYSGLYQCTAGNEAGKESCVVRVTVQYVQSIGMVAGAVTGIVAGALLIFLLVWLLIRRKDKERYEEEERPNEIREDAEAPKARLVKPSSSSSGSRSSRSGSSSTRSTANSASRSQRTLSTDAAPQPGLATQAYSLVGPEVRGSEPKKVHHANLTKAETTPSMIPSQSRAFQTV |
| 分子量 | 67.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ASAM蛋白的模拟参考文献示例(注：文献为假设性示例，实际研究需通过学术数据库查询)：
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1. **文献名称**: *Cloning and Functional Characterization of Recombinant Human ASAM in Endothelial Cell Adhesion*
**作者**: Smith J, et al.
**摘要**: 该研究成功克隆并表达重组人ASAM蛋白，发现其通过调节整合素信号通路促进内皮细胞黏附，为血管生成研究提供新靶点。
2. **文献名称**: *Structural Analysis of ASAM Reveals a Role in Immune Regulation*
**作者**: Li Y, et al.
**摘要**: 通过X射线晶体学解析重组人ASAM蛋白结构，发现其胞外结构域介导免疫细胞间相互作用，可能参与炎症反应调控。
3. **文献名称**: *ASAM Overexpression in Cancer: Recombinant Protein-Based Mechanistic Insights*
**作者**: Garcia R, et al.
**摘要**: 利用重组ASAM蛋白证明其在癌细胞迁移中的功能，揭示其与EGFR信号串扰，提示其作为肿瘤治疗潜在生物标志物。
4. **文献名称**: *ASAM Recombinant Protein Alleviates Fibrosis in Murine Models*
**作者**: Zhang H, et al.
**摘要**: 动物实验表明，重组人ASAM蛋白通过抑制TGF-β通路减缓肝纤维化进展，提示其临床转化潜力。
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**提示**：实际研究中，建议通过PubMed、Web of Science等平台以关键词“recombinant human ASAM”或“ASAM protein function”检索最新文献。ASAM的具体功能可能因细胞类型或疾病背景而异，需结合研究方向筛选。
Adhesion-associated ASAM (ASAM), also known as ACAMP, is a transmembrane glycoprotein encoded by the CLMP gene in humans. It was first identified for its role in cell-cell adhesion and tissue organization, particularly in epithelial and endothelial cells. Structurally, ASAM contains an extracellular immunoglobulin (Ig) domain, a single transmembrane helix, and a short cytoplasmic tail, sharing homology with the junctional adhesion molecule (JAM) family.
Functionally, ASAM facilitates intercellular adhesion by forming homophilic and heterophilic interactions, contributing to tight junction integrity and polarized cell architecture. Studies highlight its involvement in embryonic development, especially in cardiovascular and intestinal morphogenesis. Dysregulation of ASAM has been linked to congenital disorders like congenital short bowel syndrome (CSBS) due to CLMP mutations.
Recombinant human ASAM protein (rhASAM) is produced using expression systems (e.g., mammalian cells or *E. coli*) to study its biochemical properties, adhesion mechanisms, and signaling pathways. It serves as a critical tool for in vitro binding assays, structural analysis, and therapeutic target validation. Recent research explores its potential in regenerative medicine and modulating pathological adhesion processes in cancer metastasis or inflammatory diseases. Its recombinant form enables precise investigation of extracellular domain interactions without confounding cellular factors.
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