| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASAH3L |
| Uniprot No | Q5QJU3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-226aa |
| 氨基酸序列 | MCLFRQYATCFNSGIYLIWTLLVVVGIGSVYFHATLSFLGQMLDELAVLWVLMCALAMWFPRRYLPKIFRNDRGRFKVVVSVLSAVTTCLAFVKPAINNISLMTLGVPCTALLIAELKRCDNMRVFKLGLFSGLWWTLALFCWISDRAFCELLSSFNFPYLHCMWHILICLAAYLGCVCFAYFDAASEIPEQGPVIKFWPNEKWAFIGVPYVSLLCANKKSSVKIT |
| 分子量 | 52.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人ASAH3L蛋白的虚构示例文献(注：文献名称及作者为模拟内容，仅供格式参考)：
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1. **文献名称**: *"Recombinant human ASAH3L protein expression and enzymatic characterization"*
**作者**: Li, X. et al. (2022)
**摘要**: 报道了ASAH3L蛋白在大肠杆菌中的重组表达与纯化方法，并验证其作为神经酰胺酶的活性，证实其特异性水解特定酰基鞘氨醇的能力。
2. **文献名称**: *"ASAH3L regulates lysosomal ceramide metabolism in neurodegenerative models"*
**作者**: Gomez, R. et al. (2021)
**摘要**: 通过体外实验证明ASAH3L通过调节溶酶体鞘脂代谢影响细胞凋亡通路，重组蛋白的过表达可缓解神经元模型中的脂质累积表型。
3. **文献名称**: *"Structural insights into ASAH3L substrate specificity by cryo-EM"*
**作者**: Wang, T. et al. (2023)
**摘要**: 利用冷冻电镜解析重组ASAH3L蛋白的三维结构，揭示其底物结合口袋的关键氨基酸残基，为设计选择性抑制剂提供依据。
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如需真实文献，建议在PubMed或Web of Science中检索关键词“ASAH3L”或“ASAH3L recombinant protein”。
**Background of Recombinant Human ASAH3L Protein**
ASAH3L (N-acylsphingosine amidohydrolase 3-like) is a lysosomal enzyme belonging to the acid ceramidase family, which plays a crucial role in sphingolipid metabolism. It catalyzes the hydrolysis of acyl chains from ceramides, generating sphingosine and free fatty acids. Unlike its homolog ASAH1 (associated with Farber disease), ASAH3L exhibits substrate specificity for very-long-chain ceramides and is implicated in maintaining lipid homeostasis, particularly in cellular membranes and signaling pathways.
The ASAH3L gene is located on chromosome 11q13.2 and encodes a 385-amino-acid protein with a conserved catalytic domain. Recombinant ASAH3L is produced via heterologous expression systems (e.g., mammalian or bacterial cells) for functional studies. Its activity is pH-dependent, optimal in acidic environments resembling lysosomes.
Research links ASAH3L to neuroprotection, inflammation modulation, and cancer progression, as ceramide metabolism influences apoptosis, autophagy, and cell proliferation. Dysregulation of ASAH3L is observed in neurodegenerative disorders and某些 cancers, highlighting its therapeutic potential. Additionally, recombinant ASAH3L aids in studying enzyme deficiencies and developing targeted therapies for sphingolipid-related diseases.
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