| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARSE |
| Uniprot No | P51690 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-589aa |
| 氨基酸序列 | MLHLHHSCLCFRSWLPAMLAVLLSLAPSASSDISASRPNILLLMADDLGIGDIGCYGNNTMRTPNIDRLAEDGVKLTQHISAASLCTPSRAAFLTGRYPVRSGMVSSIGYRVLQWTGASGGPPTNETTFAKILKEKGYATGLIGKWHLGLNCESASDHCHHPLHHGFDHFYGMPFSLMGDCARWELSEKRVNLEQKLNFLFQVLALVALTLVAGKLTHLIPVSWMPVIWSALSAVLLLASSYFVGALIVHADCFLMRNHTITEQPMCFQRTTPLILQEVASFLKRNKHGPFLLFVSFLHVHIPLITMENFLGKSLHGLYGDNVEEMDWMVGRILDTLDVEGLSNSTLIYFTSDHGGSLENQLGNTQYGGWNGIYKGGKGMGGWEGGIRVPGIFRWPGVLPAGRVIGEPTSLMDVFPTVVRLADSEVPQDRVIDGQDLLPLLLGTAQHSDHEFLMHYCERFLHAARWHQRDRGTMWKVHFVTPVFQPEGAGACYGRKVCPCFGEKVVHHDPPLLFDLSRDPSETHILTPASEPVFYQVMERVQQAVWEHQRTLSPVPLQLDRLGNIWRPWLQPCCGPFPLCWCLREDDPQ |
| 分子量 | 92.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
(以下为虚构参考文献示例，仅用于演示格式)
1. **文献名称**: "SRRT interacts with the Microprocessor complex to promote miRNA biogenesis in human cells"
**作者**: Zhang Y, et al.
**摘要**: 本研究揭示SRRT蛋白通过与Microprocessor复合物(DGCR8/Drosha)相互作用，调控miRNA前体的加工过程，影响细胞分化与肿瘤发生。
2. **文献名称**: "SRRT/ARS2 is essential for neural stem cell maintenance and brain development"
**作者**: Smith JL, et al.
**摘要**: 发现SRRT在小鼠神经干细胞中高表达，敲除后导致神经发生缺陷与认知障碍，提示其通过调控RNA代谢参与神经发育。
3. **文献名称**: "A dual role of SRRT in RNA splicing and transcriptional regulation in cancer"
**作者**: Gupta R, et al.
**摘要**: 结合ChIP-seq与RNA-seq分析，证明SRRT在乳腺癌中既参与选择性剪接调控，又通过结合染色质修饰复合物影响致癌基因表达。
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**注意事项**：以上内容为示例性虚构文献，实际研究中需通过PubMed、Web of Science等数据库检索真实文献。建议搜索关键词：**SRRT/ARS2**、**RNA metabolism**、**microRNA processing**、**neural development**。
**Background of Recombinant Human Serrate RNA Effector Molecule Homolog (SRRT)**
The Serrate RNA effector molecule homolog (SRRT), also known as Ars2. is a conserved nuclear protein crucial in gene expression regulation. Initially identified in *Drosophila* for its role in Notch signaling via the Serrate ligand, mammalian SRRT has broader functions in RNA metabolism. It interacts with the nuclear cap-binding complex (CBC) and facilitates connections between transcription, RNA processing, and export. SRRT plays a dual role: it promotes the biogenesis of select microRNAs (miRNAs) and small nuclear RNAs (snRNAs) while suppressing the processing of others, ensuring precise RNA homeostasis.
SRRT is vital for cellular differentiation, proliferation, and maintaining pluripotency in stem cells. Studies link its dysregulation to cancer and neurodevelopmental disorders, highlighting its role in cell cycle control and genomic stability. As a recombinant protein, SRRT enables detailed mechanistic studies, aiding in deciphering its interactions with RNA polymerase II, spliceosomes, and export machinery. Recombinant SRRT is also explored for therapeutic potential, particularly in RNA-targeted therapies and regenerative medicine. Its multifunctional nature positions SRRT as a key molecular node integrating transcriptional and post-transcriptional processes, offering insights into disease mechanisms and novel treatment avenues.
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