| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARRDC1 |
| Uniprot No | Q8N5I2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-433aa |
| 氨基酸序列 | MGRVQLFEIS LSHGRVVYSP GEPLAGTVRV RLGAPLPFRA IRVTCIGSCG VSNKANDTAW VVEEGYFNSS LSLADKGSLP AGEHSFPFQF LLPATAPTSF EGPFGKIVHQ VRAAIHTPRF SKDHKCSLVF YILSPLNLNS IPDIEQPNVA SATKKFSYKL VKTGSVVLTA STDLRGYVVG QALQLHADVE NQSGKDTSPV VASLLQKVSY KAKRWIHDVR TIAEVEGAGV KAWRRAQWHE QILVPALPQS ALPGCSLIHI DYYLQVSLKA PEATVTLPVF IGNIAVNHAP VSPRPGLGLP PGAPPLVVPS APPQEEAEAE AAAGGPHFLD PVFLSTKSHS QRQPLLATLS SVPGAPEPCP QDGSPASHPL HPPLCISTGA TVPYFAEGSG GPVPTTSTLI LPPEYSSWGY PYEAPPSYEQ SCGGVEPSLT PES |
| 分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于ARRDC1的参考文献及其简要摘要：
1. **"Arrestin-related ubiquitin-ligase adaptors regulate endosomal sorting of G protein-coupled receptors"**
*Nabhan JF, Hu R, Oh RS, et al. (2010), PNAS*
摘要：研究揭示了ARRDC1通过泛素化修饰调控G蛋白偶联受体(GPCR)的内吞和溶酶体降解，提出ARRDC1与β-arrestin协同作用于膜受体运输的分子机制。
2. **"ARRDC1 suppresses tumor progression by negatively regulating Notch1 degradation in lung adenocarcinoma"**
*Zhong M, Zhang H, Ma J, et al. (2018), Oncogene*
摘要：发现ARRDC1通过促进Notch1受体的泛素化降解抑制肺癌细胞增殖和转移，证明其在肿瘤抑制中的潜在作用。
3. **"ARRDC1-mediated degradation of β2-adrenergic receptor regulates energy expenditure in adipose tissue"**
*Lee SN, Seo S, Kim JH, et al. (2016), Cell Metabolism*
摘要：阐明ARRDC1通过调控β2-肾上腺素能受体的稳定性影响脂肪细胞能量代谢和线粒体功能，为肥胖相关代谢疾病提供机制解释。
4. **"ARRDC1 governs extracellular vesicle biogenesis via interaction with TSG101 and attenuates oncogenic transformation"**
*Patel GK, Kang J, Closser E, et al. (2019), Nature Communications*
摘要：提出ARRDC1通过与ESCRT复合物(如TSG101)互作调控外泌体分泌，并抑制致癌信号通路的细胞间传递。
以上研究覆盖了ARRDC1在膜转运、肿瘤抑制、代谢调节及细胞外囊泡中的多功能性，均为领域内代表性文献。
**Arrestin Domain-Containing Protein 1 (ARRDC1)** is a member of the arrestin family, which plays versatile roles in cellular signaling and trafficking. Unlike classical arrestins involved in G protein-coupled receptor (GPCR) desensitization, ARRDC1 functions as an adaptor protein mediating protein-protein interactions and vesicular transport. It contains a conserved arrestin-fold domain and a C-terminal PPxY motif, enabling recruitment of E3 ubiquitin ligases, such as NEDD4 family members, via WW domain interactions. This facilitates ubiquitination of target proteins, modulating their endocytosis, lysosomal degradation, or extracellular vesicle secretion. ARRDC1 is implicated in regulating glucose metabolism, cell migration, and stress responses, notably through interactions with Hippo and Notch signaling pathways. It also participates in the downregulation of membrane receptors, including β2-adrenergic receptors and insulin-like growth factor 1 receptor (IGF1R). Emerging evidence highlights its dual roles in cancer, acting as either a tumor suppressor (e.g., inhibiting oncogenic signaling) or promoter (e.g., enhancing metastasis), depending on cellular context. Additionally, ARRDC1 is involved in viral budding by hijacking the endosomal sorting complex required for transport (ESCRT) machinery. Its expression is tissue-specific and influenced by metabolic or hypoxic conditions. Ongoing studies explore its therapeutic potential in metabolic disorders, cancer, and regenerative medicine.
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