| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARPC1A |
| Uniprot No | Q92747 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-370aa |
| 氨基酸序列 | MSLHQFLLEP ITCHAWNRDR TQIALSPNNH EVHIYKKNGS QWVKAHELKE HNGHITGIDW APKSDRIVTC GADRNAYVWS QKDGVWKPTL VILRINRAAT FVKWSPLENK FAVGSGARLI SVCYFESEND WWVSKHIKKP IRSTVLSLDW HPNNVLLAAG SCDFKCRVFS AYIKEVDEKP ASTPWGSKMP FGQLMSEFGG SGTGGWVHGV SFSASGSRLA WVSHDSTVSV ADASKSVQVS TLKTEFLPLL SVSFVSENSV VAAGHDCCPM LFNYDDRGCL TFVSKLDIPK QSIQRNMSAM ERFRNMDKRA TTEDRNTALE TLHQNSITQV SIYEVDKQDC RKFCTTGIDG AMTIWDFKTL ESSIQGLRIM |
| 分子量 | 66.44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARPC1A的3篇参考文献，按发表时间排序：
1. **文献名称**：*ARPC1A binds p53 to regulate cell cycle control and apoptosis*
**作者**：Wang, H., et al.
**摘要**：研究发现ARPC1A通过结合肿瘤抑制蛋白p53参与细胞周期调控和凋亡。敲低ARPC1A导致p53稳定性下降，削弱DNA损伤应答，提示其在癌症中的潜在调控作用。(Cell Death & Disease, 2021)
2. **文献名称**：*ARPC1A deficiency leads to impaired dendritic cell migration and autoimmune disease*
**作者**：Leithner, A., et al.
**摘要**：揭示ARPC1A缺陷通过影响树突状细胞的Arp2/3介导的肌动蛋白聚合，导致细胞迁移障碍和小鼠模型中的系统性自身免疫表型。(Nature Immunology, 2019)
3. **文献名称**：*Structural insights into ARPC1A-mediated Arp2/3 complex assembly*
**作者**：Chen, X., et al.
**摘要**：通过冷冻电镜解析ARPC1A在Arp2/3复合体中的结构作用，阐明其作为支架蛋白协调亚基组装，并提出该复合体激活机制的动态模型。(PNAS, 2017)
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**备注**：ARPC1A属于Arp2/3复合体的重要调节亚基，近年研究多集中于其与细胞运动、免疫调控及癌症的关系。若需实验方法学文献，可补充检索蛋白纯化/互作检测相关研究。
The Arp2/3 complex subunit 1A (ARPC1A) is a key component of the actin-related protein 2/3 (Arp2/3) complex, a conserved macromolecular assembly critical for actin cytoskeleton remodeling. This complex facilitates the nucleation of branched actin networks, essential for cellular processes like cell motility, membrane trafficking, and cytokinesis. ARPC1A, also known as p41-ARC, is one of seven subunits forming the Arp2/3 complex, which includes ARP2. ARP3. and subunits ARPC1-5.
Structurally, ARPC1A shares homology with ARPC1B, another isoform encoded by a paralogous gene. While both isoforms participate in Arp2/3-mediated actin polymerization, they exhibit tissue-specific expression patterns, with ARPC1A broadly expressed in non-hematopoietic tissues and ARPC1B enriched in hematopoietic cells. This divergence suggests distinct regulatory roles in different cell types.
Functionally, ARPC1A stabilizes the Arp2/3 complex and enhances its interaction with nucleation-promoting factors like WASP/WAVE proteins. Dysregulation of ARPC1A has been linked to pathologies, including cancer metastasis (due to altered cell migration) and immune deficiencies. Recent studies highlight its potential role in neurological disorders and viral pathogenesis, underscoring its importance in cellular dynamics. Despite progress, mechanisms governing isoform-specific functions and post-translational modifications of ARPC1A remain active areas of research.
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