| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARR3 |
| Uniprot No | P36575 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-388aa |
| 氨基酸序列 | MSKVFKKTSS NGKLSIYLGK RDFVDHVDTV EPIDGVVLVD PEYLKCRKLF VMLTCAFRYG RDDLEVIGLT FRKDLYVQTL QVVPAESSSP QGPLTVLQER LLHKLGDNAY PFTLQMVTNL PCSVTLQPGP EDAGKPCGID FEVKSFCAEN PEETVSKRDY VRLVVRKVQF APPEAGPGPS AQTIRRFLLS AQPLQLQAWM DREVHYHGEP ISVNVSINNC TNKVIKKIKI SVDQITDVVL YSLDKYTKTV FIQEFTETVA ANSSFSQSFA VTPILAASCQ KRGLALDGKL KHEDTNLASS TIIRPGMDKE LLGILVSYKV RVNLMVSCGG ILGDLTASDV GVELPLVLIH PKPSHEAASS EDIVIEEFTR KGEEESQKAV EAEGDEGS |
| 分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ARR3(Arrestin-C)的3篇文献参考示例(虚拟信息供格式参考)：
1. **标题**: *"Crystal structure of human cone arrestin (ARR3) reveals functional insights into retinoid binding and phototransduction regulation"*
**作者**: Li, X., Smith, T., Chen, R.
**摘要**: 本研究解析了重组表达的人ARR3蛋白晶体结构，揭示了其视黄酸结合位点及参与光信号传导调控的关键结构域，为视网膜疾病机制提供分子基础。
2. **标题**: *"Recombinant expression and functional characterization of ARR3 in retinal Müller cells"*
**作者**: Zhang, S., Wang, L., Kim, J. et al.
**摘要**: 开发了AR3重组蛋白的哺乳细胞表达系统，验证其在体外抑制GPCR信号通路的活性，并发现其对视网膜胶质细胞光损伤具有保护作用。
3. **标题**: *"ARR3 mutations cause X-linked high myopia through disrupted protein interactions with GRK7"*
**作者**: Ma, C., Zhou, Y., Thompson, D.A.
**摘要**: 通过筛选高度近视家系发现ARR3基因突变可破坏重组ARR3蛋白与GRK7激酶的相互作用，导致光信号异常传导及眼部发育缺陷。
(注：以上为虚拟文献示例，实际研究可检索PubMed使用关键词 "ARR3"、"X-arrestin" 或 "cone arrestin" 获取)
Recombinant human Arrestin-C (ARR3), also known as β-arrestin 2. is a ubiquitously expressed cytosolic protein critical for regulating G protein-coupled receptor (GPCR) signaling. As a member of the arrestin family, it plays a dual role in both desensitizing activated GPCRs by sterically blocking G protein coupling and facilitating receptor internalization via clathrin-coated pits. Beyond signal termination, ARR3 serves as a scaffold, linking receptors to downstream signaling pathways like MAPK/ERK, contributing to cellular processes such as proliferation, migration, and apoptosis. Its structural features, including N- and C-terminal domains, enable conformational changes upon receptor phosphorylation, ensuring binding specificity.
The recombinant form of ARR3 is engineered using expression systems (e.g., E. coli, mammalian cells) for high-purity, functional studies. This allows precise exploration of its mechanisms in receptor trafficking, biased signaling, and non-canonical pathways. Dysregulation of ARR3 is implicated in pathologies such as cancer, cardiovascular diseases, and neurological disorders, making it a therapeutic target. In cancer, for example, ARR3 modulates metastasis-related signaling, while in neurodegeneration, it affects amyloid-beta receptor dynamics. Recombinant ARR3 is widely utilized in drug discovery, especially for biased ligands that selectively activate G protein- or arrestin-mediated effects. Its versatility in structural and functional research underpins advances in understanding GPCR biology and developing targeted therapies.
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