| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARPM1 |
| Uniprot No | Q9BYD9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-372aa |
| 氨基酸序列 | MNHCQLPVVIDNGSGMIKAGVAGCREPQFIYPNIIGRAKGQSRAAQGGLELCVGDQAQDWRSSLFISYPVERGLITSCEDMEIMWKHIYDYNLKLKPCDGPVLITEPALNPLANRQQITEMFFEHLGVPAFYMSIQAVLALFAAGFTTGLVLNSGAGVTQSVPIFEGYCLPHGVQQLDLAGLDLTNYLMVLMKNHGIMLLSASDRKIVEDIKESFCYVAMNYEEEMAKKPDCLEKVYQLPDGKVIQLHDQLFSCPEALFSPCHMNLEAPGIDKICFSSIMKCDTGLRNSFFSNIILAGGSTSFPGLDKRLVKDIAKVAPANTAVQVIAPPERKISVWMGGSILASLSAFQDMWITAAEFKEVGPNIVHQRCF |
| 分子量 | 67.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人相关蛋白M1(ARPM1)的虚构参考文献示例(假设性内容，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *Structural characterization and functional analysis of recombinant human ARPM1 protein*
**作者**: Zhang Y, Liu T, Wang X.
**摘要**: 本研究通过X射线晶体学解析了ARPM1的三维结构，揭示其与ATP结合的活性位点，并通过体外实验验证了其对细胞骨架重塑的调控功能。
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2. **文献名称**: *ARPM1 promotes tumor metastasis via modulating the TGF-β signaling pathway*
**作者**: Chen L, et al.
**摘要**: 文章发现ARPM1在多种癌症组织中高表达，并通过激活TGF-β通路促进上皮-间质转化(EMT)和肿瘤细胞侵袭迁移，抑制ARPM1可显著降低转移风险。
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3. **文献名称**: *Expression and purification of biologically active recombinant ARPM1 in E. coli*
**作者**: Gupta S, et al.
**摘要**: 开发了一种高效的大肠杆菌表达系统用于重组ARPM1生产，优化纯化步骤后获得高纯度蛋白，并证明其在体外具有抗炎活性。
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4. **文献名称**: *ARPM1 deficiency leads to impaired neuronal development in zebrafish models*
**作者**: Lee JH, et al.
**摘要**: 利用斑马鱼模型发现ARPM1基因敲除导致神经元突触形成异常，提示其在神经系统发育中可能发挥关键作用。
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**提示**：若需真实文献，建议通过PubMed、Google Scholar等平台检索全称或相关关键词(如"ARPM1"或扩展名)，并核对蛋白名称的准确性(可能存在拼写差异)。
Recombinant human-associated protein M1 (ARPM1) is a genetically engineered protein derived from human cellular components, designed to mimic the structure and function of the endogenous protein. ARPM1 is implicated in various cellular processes, including intracellular signaling, enzymatic regulation, and interactions with other biomolecules such as DNA, RNA, or partner proteins. Its exact physiological role remains under investigation, but preliminary studies suggest involvement in pathways related to cellular homeostasis, immune response modulation, and possibly oncogenesis. Structurally, ARPM1 typically features conserved domains critical for ligand binding or catalytic activity, although specific motifs may vary depending on splice variants or post-translational modifications.
The recombinant form is produced using expression systems (e.g., E. coli, yeast, or mammalian cells) followed by purification techniques to ensure bioactivity and low endotoxin levels. This allows researchers to study its biochemical properties, develop diagnostic tools, or explore therapeutic applications. ARPM1 has gained attention in drug discovery due to its potential as a target for small-molecule inhibitors or biologics, particularly in diseases like cancer or autoimmune disorders where its dysregulation has been observed. Current research focuses on elucidating its interactome, structural dynamics, and role in disease models. Challenges include optimizing recombinant yield, ensuring proper folding, and validating in vivo relevance. As a tool protein, ARPM1 also aids in antibody development and mechanistic studies of related pathways.
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