| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARSK |
| Uniprot No | Q6UWY0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-536aa |
| 氨基酸序列 | MLLLWVSVVAALALAVLAPGAGEQRRRAAKAPNVVLVVSDSFDGRLTFHPGSQVVKLPFINFMKTRGTSFLNAYTNSPICCPSRAAMWSGLFTHLTESWNNFKGLDPNYTTWMDVMERHGYRTQKFGKLDYTSGHHSISNRVEAWTRDVAFLLRQEGRPMVNLIRNRTKVRVMERDWQNTDKAVNWLRKEAINYTEPFVIYLGLNLPHPYPSPSSGENFGSSTFHTSLYWLEKVSHDAIKIPKWSPLSEMHPVDYYSSYTKNCTGRFTKKEIKNIRAFYYAMCAETDAMLGEIILALHQLDLLQKTIVIYSSDHGELAMEHRQFYKMSMYEASAHVPLLMMGPGIKAGLQVSNVVSLVDIYPTMLDIAGIPLPQNLSGYSLLPLSSETFKNEHKVKNLHPPWILSEFHGCNVNASTYMLRTNHWKYIAYSDGASILPQLFDLSSDPDELTNVAVKFPEITYSLDQKLHSIINYPKVSASVHQYNKEQFIKWKQSIGQNYSNVIANLRWHQDWQKEPRKYENAIDQWLKTHMNPRAV |
| 分子量 | 87.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条与重组人芳基硫酸酯酶K(ARSK)相关的参考文献示例及简要概括：
1. **文献名称**: "Recombinant human aryl sulfatase K: enzymatic characterization and substrate specificity analysis"
**作者**: Tanaka H. et al.
**摘要**: 研究报道了通过哺乳动物细胞表达系统成功重组生产ARSK，并分析了其对不同硫酸酯类底物的催化效率，揭示了ARSK在黏多糖代谢中的选择性作用。
2. **文献名称**: "Crystal structure of human ARSK reveals molecular basis of sulfatase activity in lysosomal storage disorders"
**作者**: Schmidt B. et al.
**摘要**: 通过X射线晶体学解析了ARSK的三维结构，确定了其活性位点关键氨基酸残基，解释了ARSK功能缺陷与特定溶酶体贮积症病理机制的关联。
3. **文献名称**: "ARSK deficiency in mice induces a novel form of mucopolysaccharidosis: implications for diagnostics and therapeutic development"
**作者**: Gortz F. et al.
**摘要**: 构建ARSK基因敲除小鼠模型，发现其表现出类似人类黏多糖贮积症的病理特征，为开发重组ARSK酶替代疗法提供了临床前依据。
(注：上述文献为示例性概括，实际引用需根据具体发表论文调整。)
Recombinant human arylsulfatase K (ARSK) is a lysosomal sulfatase enzyme involved in the hydrolysis of sulfate esters from various substrates, particularly glycosaminoglycans (GAGs) and other sulfated biomolecules. ARSK belongs to the sulfatase family, which plays critical roles in maintaining cellular homeostasis through GAG degradation. It specifically targets 2-sulfoglucuronate residues in heparan sulfate and dermatan sulfate, contributing to the stepwise breakdown of these complex polysaccharides. The enzyme is encoded by the ARSK gene located on human chromosome 5q11-q13.
Deficiencies in sulfatase enzymes, including ARSK, are linked to lysosomal storage disorders (LSDs) characterized by GAG accumulation, though ARSK-specific pathologies remain less characterized compared to related sulfatases like ARSB or ARSE. Recent studies suggest its potential involvement in connective tissue metabolism and sulfur detoxification pathways. Recombinant ARSK is produced using biotechnological systems (e.g., mammalian or microbial cell cultures) for research and therapeutic applications. Its recombinant form enables detailed structural-functional studies and exploration of enzyme replacement therapies (ERT) for rare metabolic diseases. Current research focuses on elucidating substrate specificity, catalytic mechanisms, and pathophysiological roles, providing insights into broader sulfatase biology and targeted treatment strategies for LSDs.
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