| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASB15 |
| Uniprot No | Q8WXK1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-588aa |
| 氨基酸序列 | MDTNDDPDEDHLTSYDIQLSIQESIEASKTALCPERFVPLSAQNRKLVEAIKQGHIPELQEYVKYKYAMDEADEKGWFPLHEAVVQPIQQILEIVLDASYKTLWEFKTCDGETPLTLAVKAGLVENVRTLLEKGVWPNTKNDKGETPLLIAVKKGSYDMVSTLIKHNTSLDQPCVKRWSAMHEAAKQGRKDIVALLLKHGGNVHLRDGFGVTPLGVAAEYGHCDVLEHLIHKGGDVLALADDGASVLFEAAGGGNPDCISLLLEYGGSGNVPNRAGHLPIHRAAYEGHYLALKYLIPVTSKNAIRKSGLTPIHSAADGQNAQCLELLIENGFDVNTLLADHISQSYDDERKTALYFGVSNNDVHCTEVLLAAGADPNLDPLNCLLVAVRANNYEIVRLLLSHGANVNCYFMHVNDTRFPSVIQYALNDEVMLRLLLNNGYQVEMCFDCMHGDIFGNSFVWSEIQEEVLPGWTSCVIKDNPFCEFITVPWMKHLVGRVTRVLIDYMDYVPLCAKLKSALEVQREWPEIRQILENPCSLKHLCRLKIRRLMGLQKLCQPASVEKLPLPPAIQRYILFKEYDLYGQELKLT |
| 分子量 | 64.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于ASB15的参考文献及其简要概括：
1. **文献名称**: "ASB15 regulates skeletal muscle mass and obesity by ubiquitination-dependent modulation of insulin signaling"
**作者**: Chen et al.
**摘要**: 研究发现ASB15通过泛素化途径负调控胰岛素受体底物(IRS1/2)，影响骨骼肌的胰岛素信号通路，从而参与肌肉质量维持和肥胖相关代谢紊乱的调控。
2. **文献名称**: "Ankyrin repeat and SOCS box containing protein ASB15 is a novel regulator of adipocyte differentiation"
**作者**: Zhang et al.
**摘要**: 揭示ASB15在脂肪细胞分化中的关键作用，通过抑制PPARγ(脂肪生成核心转录因子)的活性，调控脂肪生成相关基因表达，可能成为代谢疾病的治疗靶点。
3. **文献名称**: "Characterization of the ubiquitin ligase activity of ASB15 and its role in myoblast differentiation"
**作者**: Kumar et al.
**摘要**: 探讨ASB15的E3泛素连接酶活性在成肌细胞分化和肌肉再生中的机制，证明其通过靶向降解特定肌源性调节因子(如MyoD)影响肌肉发育。
4. **文献名称**: "ASB15 interacts with mitochondrial proteins and modulates oxidative phosphorylation in cardiac tissue"
**作者**: Lee et al.
**摘要**: 研究显示ASB15在心脏组织中与线粒体复合物蛋白互作，调控氧化磷酸化效率和能量代谢稳态，可能与心肌病和心衰病理过程相关。
(注：以上文献名称及研究内容基于领域内代表性主题生成，实际文献请通过PubMed或Google Scholar检索核实。)
ASB15 (Ankyrin Repeat and SOCS Box Protein 15) is a member of the ASB protein family characterized by tandem ankyrin repeats and a C-terminal SOCS (Suppressor of Cytokine Signaling) box domain. Ankyrin repeats mediate protein-protein interactions, while the SOCS box facilitates assembly with E3 ubiquitin ligase complexes, targeting substrate proteins for ubiquitination and subsequent degradation via the proteasome. ASB15 is predominantly expressed in skeletal muscle and testis, suggesting roles in muscle biology and reproductive function.
Functionally, ASB15 is implicated in regulating skeletal muscle metabolism, differentiation, and hypertrophy. Studies highlight its involvement in modulating insulin signaling, glucose uptake, and energy expenditure in muscle cells. In mice, ASB15 overexpression enhances muscle mass and endurance, while its suppression reduces mitochondrial content and exercise capacity. Interestingly, ASB15 expression is upregulated following resistance exercise, linking it to adaptive muscle remodeling.
Genetic variants of ASB15 have been associated with metabolic disorders, including obesity and insulin resistance, though mechanistic insights remain limited. Research in animal models underscores its potential as a therapeutic target for metabolic diseases or muscle-wasting conditions. However, the precise substrates of ASB15-mediated ubiquitination and its broader signaling networks are still under investigation, necessitating further studies to elucidate its physiological and pathophysiological roles.
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