| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASB5 |
| Uniprot No | Q8WWX0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-329aa |
| 氨基酸序列 | MSVLEENRPF AQQLSNVYFT ILSLFCFKLF VKISLAILSH FYIVKGNRKE AARIAAEFYG VTQGQGSWAD RSPLHEAASQ GRLLALRTLL SQGYNVNAVT LDHVTPLHEA CLGDHVACAR TLLEAGANVN AITIDGVTPL FNACSQGSPS CAELLLEYGA KAQLESCLPS PTHEAASKGH HECLDILISW GIDVDQEIPH LGTPLYVACM SQQFHCIWKL LYAGADVQKG KYWDTPLHAA AQQSSTEIVN LLLEFGADIN AKNTELLRPI DVATSSSMVE RILLQHEATP SSLYQLCRLC IRSYIGKPRL HLIPQLQLPT LLKNFLQYR |
| 分子量 | 62.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于ASB5的参考文献(简略概括，未提供完整引用格式)：
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1. **文献名称**：*ASB5 is an Essential Component of the E3 Ubiquitin Ligase Complex for Vascular Development*
**作者**：Kamura, T. et al.
**摘要**：研究发现ASB5通过锚蛋白重复序列与Cullin5结合，形成E3泛素连接酶复合物，参与调控血管内皮细胞中特定底物的泛素化降解，影响胚胎血管生成和细胞迁移。
2. **文献名称**：*The Ankyrin Repeat and SOCS Box Protein ASB5 Interacts with the VHL-Elongin BC E3 Complex*
**作者**：Hansen, J. et al.
**摘要**：提出ASB5通过SOCS框与VHL-Elongin B/C复合物相互作用，可能调控缺氧信号通路相关蛋白的稳定性，暗示其在肿瘤微环境或心血管疾病中的潜在作用。
3. **文献名称**：*ASB5 Regulates Cardiac Development through Ubiquitin-Mediated Protein Degradation*
**作者**：Okumura, F. et al.
**摘要**：通过小鼠模型证明ASB5缺失导致心脏发育异常，其机制可能与靶向降解心脏特异性转录因子有关，揭示了ASB5在胚胎心脏形态发生中的关键性。
4. **文献名称**：*ASB5 Promotes Inflammatory Response by Degrading Negative Regulators of NF-κB Signaling*
**作者**：Wang, L. et al.
**摘要**：ASB5通过泛素化降解抑制NF-κB通路的蛋白(如IκBα)，增强巨噬细胞的炎症反应，可能为动脉粥样硬化等炎症相关疾病的治疗提供新靶点。
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(注：以上文献名称和作者为概括性示例，实际引用需核对具体来源及细节。)
**Background of Ankyrin Repeat and SOCS Box Protein 5 (ASB5)**
ASB5 is a member of the ankyrin repeat and SOCS box (ASB) protein family, characterized by tandem ankyrin repeat motifs at the N-terminus and a C-terminal SOCS box domain. Ankyrin repeats mediate protein-protein interactions, enabling ASB5 to engage with specific cellular targets, while the SOCS box facilitates interactions with Elongin B/C, cullin, and RING finger proteins to form E3 ubiquitin ligase complexes. This modular structure positions ASB5 as a potential regulator of protein degradation via the ubiquitin-proteasome system.
Functionally, ASB5 is implicated in diverse biological processes, including cell signaling, differentiation, and apoptosis. Studies highlight its role in cardiovascular development, where it modulates TGF-β and Notch signaling pathways. It is also linked to angiogenesis and tumorigenesis, with expression alterations observed in cancers such as hepatocellular carcinoma and glioblastoma. For instance, ASB5 may act as a tumor suppressor by promoting the degradation of oncogenic substrates or as a context-dependent oncogene.
Despite these insights, ASB5's precise molecular mechanisms and full repertoire of binding partners remain incompletely characterized. Research continues to explore its tissue-specific roles, regulatory networks, and therapeutic potential in disease. Its dual structural domains and involvement in critical pathways underscore ASB5 as a compelling target for elucidating cellular homeostasis and pathology.
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