| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASCC2 |
| Uniprot No | Q9H1I8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-757aa |
| 氨基酸序列 | MPALPLDQLQITHKDPKTGKLRTSPALHPEQKADRYFVLYKPPPKDNIPALVEEYLERATFVANDLDWLLALPHDKFWCQVIFDETLQKCLDSYLRYVPRKFDEGVASAPEVVDMQKRLHRSVFLTFLRMSTHKESKDHFISPSAFGEILYNNFLFDIPKILDLCVLFGKGNSPLLQKMIGNIFTQQPSYYSDLDETLPTILQVFSNILQHCGLQGDGANTTPQKLEERGRLTPSDMPLLELKDIVLYLCDTCTTLWAFLDIFPLACQTFQKHDFCYRLASFYEAAIPEMESAIKKRRLEDSKLLGDLWQRLSHSRKKLMEIFHIILNQICLLPILESSCDNIQGFIEEFLQIFSSLLQEKRFLRDYDALFPVAEDISLLQQASSVLDETRTAYILQAVESAWEGVDRRKATDAKDPSVIEEPNGEPNGVTVTAEAVSQASSHPENSEEEECMGAAAAVGPAMCGVELDSLISQVKDLLPDLGEGFILACLEYYHYDPEQVINNILEERLAPTLSQLDRNLDREMKPDPTPLLTSRHNVFQNDEFDVFSRDSVDLSRVHKGKSTRKEENTRSLLNDKRAVAAQRQRYEQYSVVVEEVPLQPGESLPYHSVYYEDEYDDTYDGNQVGANDADSDDELISRRPFTIPQVLRTKVPREGQEEDDDDEEDDADEEAPKPDHFVQDPAVLREKAEARRMAFLAKKGYRHDSSTAVAGSPRGHGQSRETTQERRKKEANKATRANHNRRTMADRKRSKGMIPS |
| 分子量 | 112.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASCC2的3篇虚拟参考文献(示例框架，实际文献需通过学术数据库查询)：
1. **标题**：*ASCC2 regulates DNA damage repair through modulating ubiquitination signaling*
**作者**：Zhang Y, et al.
**摘要**：研究发现ASCC2作为ASC-1复合物亚基，在DNA损伤修复中通过去泛素化调控关键修复蛋白稳定性，缺失ASCC2导致基因组不稳定性和肿瘤易感性增加。
2. **标题**：*ASCC2 promotes tumor progression via NF-κB signaling in colorectal cancer*
**作者**：Wang L, et al.
**摘要**：在结直肠癌中，ASCC2通过结合NF-κB增强其转录活性，促进肿瘤细胞增殖和侵袭，提示ASCC2可作为潜在治疗靶点。
3. **标题**：*Structural and functional characterization of ASCC2 in transcriptional coactivation*
**作者**：Smith J, et al.
**摘要**：通过结构生物学分析揭示了ASCC2与RNA聚合酶II的相互作用机制，证明其在激活基因转录中的关键调控作用，为理解复合物组装提供新见解。
**提示**：以上为模拟示例，实际文献建议通过 **PubMed/Google Scholar** 搜索关键词“ASCC2”或“ASC-1 complex subunit 2”获取最新研究，注意筛选近五年高引论文以确保前沿性。
ASCC2 (Activating Signal Cointegrator 1 Complex Subunit 2) is a critical component of the ASC-1 (Activating Signal Cointegrator-1) complex, which also includes ASCC1. ASCC3. and TRIP4. This evolutionarily conserved multiprotein complex plays diverse roles in transcriptional regulation, DNA damage repair, and mRNA processing. Structurally, ASCC2 contains an OB-fold domain, facilitating interactions with nucleic acids or partner proteins.
Functionally, the ASC-1 complex is recruited to sites of DNA alkylation damage, where ASCC2 contributes to transcription-coupled repair by displacing stalled RNA polymerase II and promoting lesion resolution. It also participates in regulating ribosomal RNA biogenesis and mRNA splicing through interactions with spliceosome components. Recent studies highlight its involvement in resolving R-loop formation, maintaining genomic stability.
ASCC2 dysregulation has been linked to cancer progression and therapy resistance. Reduced ASCC2 expression correlates with poor prognosis in lung adenocarcinoma and hepatocellular carcinoma, potentially due to impaired DNA repair mechanisms. Additionally, ASCC2 mutations are associated with developmental disorders characterized by neurological abnormalities, suggesting its importance in neurogenesis. Emerging evidence implicates ASCC2 in metabolic regulation, particularly lipid metabolism, through transcriptional coactivation of nuclear receptors. Its dual roles in genome maintenance and gene expression regulation position ASCC2 as a molecular nexus connecting chromatin dynamics to cellular homeostasis.
×
