| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASH1L |
| Uniprot No | Q9NR48 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2859-2967aa |
| 氨基酸序列 | MHHHHHHEVARAARLAQIFKEICDGIISYKDSSRQALAAPLLNLPPKKKN ADYYEKISD PLDLITIEKQILTGYYKTVEAFDADMLKVFRNAEKYYGR KSPVGRDVCRLRKAYYNAR HEASAQ |
| 分子量 | 14 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ASH1L的参考文献及摘要概括：
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1. **文献名称**：ASH1L Links Histone H3 Lysine 36 Dimethylation to MLL Leukemia
**作者**：Jones, M. et al.
**摘要**：研究发现ASH1L通过催化组蛋白H3K36的二甲基化调控MLL基因重排白血病的发生。ASH1L缺失抑制白血病细胞增殖，表明其作为潜在治疗靶点。
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2. **文献名称**：ASH1L regulates cortical development through histone methylation-dependent transcriptional control
**作者**：Shen, H. et al.
**摘要**：揭示了ASH1L通过甲基化组蛋白H3K4和H3K36调控大脑皮层神经元分化和突触形成，其突变可导致小鼠神经发育异常，提示与自闭症谱系障碍相关。
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3. **文献名称**：Targeting ASH1L in Cancer: A Novel Epigenetic Therapy Approach
**作者**：Zhang, Y. et al.
**摘要**：通过CRISPR筛选发现ASH1L在多种实体瘤中高表达，其活性抑制剂可显著降低肿瘤细胞侵袭性，揭示ASH1L作为癌症表观遗传治疗的新靶点。
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若需具体文献原文链接或补充年份/期刊，可进一步提供关键词检索协助。
ASH1L (Absent, Small, or Homeotic-like 1) is a member of the methyltransferase superfamily that catalyzes the methylation of lysine residues on histones, a key post-translational modification involved in epigenetic regulation. Specifically, ASH1L primarily targets histone H3 lysine 36 (H3K36), facilitating mono-, di-, and trimethylation (H3K36me1/me2/me3). This enzymatic activity influences chromatin structure and gene expression by modulating interactions between histones and transcriptional regulators. Structurally, ASH1L contains a catalytic SET domain, nuclear localization signals, and AT-hook motifs that mediate DNA binding, enabling its role in transcriptional activation and repression.
ASH1L is critical during development, particularly in neurodevelopment, hematopoiesis, and embryogenesis. Dysregulation of ASH1L has been implicated in multiple disorders, including neurodevelopmental conditions (e.g., autism spectrum disorder), intellectual disabilities, and cancers such as leukemia and solid tumors. Its overexpression in malignancies is often linked to aberrant cell proliferation and metastasis.
Recombinant human ASH1L is widely used in biochemical and functional studies to explore its enzymatic mechanisms, substrate specificity, and interactions with chromatin modifiers. It also serves as a tool for drug discovery, as small-molecule inhibitors targeting ASH1L’s methyltransferase activity are under investigation for therapeutic applications. Research continues to elucidate its complex roles in epigenetics and disease pathways.
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