| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP5G2 |
| Uniprot No | Q06055 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-141aa |
| 氨基酸序列 | MFACSKFVSTPSLVKSTSQLLSRPLSAVVLKRPEILTDESLSSLAVSCPLTSLVSSRSFQTSAISRDIDTAAKFIGAGAATVGVAGSGAGIGTVFGSLIIGYARNPSLKQQLFSYAILGFALSEAMGLFCLMVAFLILFAM |
| 分子量 | 41.25 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人(ATP5G2)蛋白的3篇代表性文献示例(内容基于潜在研究方向模拟)：
1. **文献名称**：**“ATP5G2 regulates mitochondrial metabolism and oncogenic transformation via modulating ATP synthase activity”**
**作者**：Zhang et al.
**摘要**：研究阐明了ATP5G2作为线粒体ATP合酶的关键亚基，通过重组蛋白技术证实其通过调控ATP合酶活性影响细胞能量代谢，并在肿瘤细胞的异常增殖和代谢重编程中发挥重要作用。
2. **文献名称**：**“Structural and functional characterization of recombinant human ATP5G2 in lipid membrane binding”**
**作者**：Lee et al.
**摘要**：利用大肠杆菌表达系统纯化重组人ATP5G2蛋白，结合冷冻电镜技术解析其三维结构，揭示其与线粒体膜脂质互作的分子机制，为ATP合酶组装提供结构基础。
3. **文献名称**：**“ATP5G2 knockout mice exhibit mitochondrial dysfunction and cardiac hypertrophy linked to altered proton transport”**
**作者**：Wang et al.
**摘要**：通过构建ATP5G2基因敲除小鼠模型，结合重组蛋白回补实验，证明ATP5G2缺失导致线粒体质子通道功能异常，引发心肌肥厚和能量代谢障碍。
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**注**：以上文献为虚拟示例，实际研究中请通过PubMed或Google Scholar检索真实文献(关键词：ATP5G2/ATP synthase subunit C/重组表达)。
The recombinant human ATP5G2 protein, also known as ATP synthase membrane subunit 6.2 or ATP synthase subunit c 2. is a critical component of the mitochondrial ATP synthase complex (Complex V). This protein is encoded by the nuclear gene ATP5G2. one of three isoforms (ATP5G1. G2. G3) of the subunit c family that form the proteolipid ring within the F0 domain of ATP synthase. As part of the F0 channel, ATP5G2 facilitates proton translocation across the mitochondrial inner membrane, driving ATP synthesis in the F1 domain via a rotary mechanism. The mature protein consists of approximately 75 amino acids, featuring two transmembrane helices connected by a hydrophilic loop.
Recombinant ATP5G2 is produced using genetic engineering techniques for functional and structural studies. Its deregulation has been implicated in metabolic disorders, neurodegenerative diseases (e.g., Parkinson's), and cancer, where mitochondrial bioenergetics are often compromised. As ATP synthase subunits are essential for cellular energy homeostasis, recombinant ATP5G2 serves as a valuable tool for investigating disease mechanisms, drug screening targeting mitochondrial dysfunction, and deciphering proton translocation dynamics. Its overexpression or mutations may affect mitochondrial membrane potential and cellular apoptosis pathways, highlighting therapeutic research potential.
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