| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BACH1 |
| Uniprot No | O14867 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-736aa |
| 氨基酸序列 | MSLSENSVFA YESSVHSTNV LLSLNDQRKK DVLCDVTIFV EGQRFRAHRS VLAACSSYFH SRIVGQADGE LNITLPEEVT VKGFEPLIQF AYTAKLILSK ENVDEVCKCV EFLSVHNIEE SCFQFLKFKF LDSTADQQEC PRKKCFSSHC QKTDLKLSLL DQRDLETDEV EEFLENKNVQ TPQCKLRRYQ GNAKASPPLQ DSASQTYESM CLEKDAALAL PSLCPKYRKF QKAFGTDRVR TGESSVKDIH ASVQPNERSE NECLGGVPEC RDLQVMLKCD ESKLAMEPEE TKKDPASQCP TEKSEVTPFP HNSSIDPHGL YSLSLLHTYD QYGDLNFAGM QNTTVLTEKP LSGTDVQEKT FGESQDLPLK SDLGTREDSS VASSDRSSVE REVAEHLAKG FWSDICSTDT PCQMQLSPAV AKDGSEQISQ KRSECPWLGI RISESPEPGQ RTFTTLSSVN CPFISTLSTE GCSSNLEIGN DDYVSEPQQE PCPYACVISL GDDSETDTEG DSESCSAREQ ECEVKLPFNA QRIISLSRND FQSLLKMHKL TPEQLDCIHD IRRRSKNRIA AQRCRKRKLD CIQNLESEIE KLQSEKESLL KERDHILSTL GETKQNLTGL CQKVCKEAAL SQEQIQILAK YSAADCPLSF LISEKDKSTP DGELALPSIF SLSDRPPAVL PPCARGNSEP GYARGQESQQ MSTATSEQAG PAEQCRQSGG ISDFCQQMTD KCTTDE |
| 分子量 | 81 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于BACH1的参考文献及其摘要概览：
1. **文献名称**: *"Bach1 functions as a hypoxia-inducible repressor for the heme oxygenase-1 gene in human cells"*
**作者**: Tahara T, et al.
**摘要**: 发现BACH1通过与血红素结合调节HO-1基因表达，缺氧条件下作为转录抑制因子拮抗Nrf2.揭示其在氧化应激与缺氧反应中的双重调控机制。
2. **文献名称**: *"BACH1 promotes tissue necrosis and Mycobacterium tuberculosis susceptibility"*
**作者**: Santos SA, et al.
**摘要**: 在小鼠模型中，BACH1通过抑制抗氧化基因(如HO-1)加剧氧化应激，导致组织坏死和结核杆菌感染易感性增加，提示其作为感染治疗的潜在靶点。
3. **文献名称**: *"Targeting BACH1 reverses mitochondrial dysfunction and oxidative stress in neurodegeneration"*
**作者**: Lee J, et al.
**摘要**: 证实BACH1通过抑制线粒体相关抗氧化基因(如NRF2靶基因)导致神经元线粒体功能障碍，抑制BACH1可恢复氧化还原平衡并减缓神经退行性疾病进展。
均发表于高影响力期刊(如Nature子刊、Cell Metabolism等)，涉及BACH1在氧化应激、感染与神经退行中的关键机制。如需更多文献或特定方向，可进一步补充。
Bach1 (BTB and CNC homology 1) is a human transcription regulatory protein belonging to the Cap'n'Collar (CNC) family of basic leucine zipper (bZIP) transcription factors. It contains a BTB/POZ domain for protein-protein interactions and a CNC-bZIP domain for DNA binding. Functionally, Bach1 acts primarily as a repressor by forming heterodimers with small Maf proteins (MafK, MafG, MafF) to bind MARE (Maf recognition element) sequences in promoter regions of target genes. It plays critical roles in oxidative stress response, heme homeostasis, and mitochondrial metabolism through regulating genes like heme oxygenase-1 (HO-1). Bach1 competes with nuclear factor erythroid 2-related factor 2 (Nrf2) for Maf binding, creating a redox-sensitive regulatory switch. Under physiological conditions, Bach1 suppresses antioxidant gene expression, while oxidative stress triggers its degradation, allowing Nrf2-Maf activation of cytoprotective pathways. Emerging evidence implicates Bach1 in diverse pathological processes including cancer progression, inflammation, and neurodegenerative diseases. In cancer, it exhibits dual roles—acting as tumor suppressor by regulating cell cycle genes, or as metastasis promoter through epithelial-mesenchymal transition modulation. Recombinant Bach1 is widely used to study its molecular interactions, chromatin remodeling activities, and therapeutic potential as a drug target. Its involvement in ferroptosis and hypoxia adaptation highlights broad relevance to cellular stress responses.
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