| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BACH |
| Uniprot No | O14867 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-736aa |
| 氨基酸序列 | MSLSENSVFA YESSVHSTNV LLSLNDQRKK DVLCDVTIFV EGQRFRAHRS VLAACSSYFH SRIVGQADGE LNITLPEEVT VKGFEPLIQF AYTAKLILSK ENVDEVCKCV EFLSVHNIEE SCFQFLKFKF LDSTADQQEC PRKKCFSSHC QKTDLKLSLL DQRDLETDEV EEFLENKNVQ TPQCKLRRYQ GNAKASPPLQ DSASQTYESM CLEKDAALAL PSLCPKYRKF QKAFGTDRVR TGESSVKDIH ASVQPNERSE NECLGGVPEC RDLQVMLKCD ESKLAMEPEE TKKDPASQCP TEKSEVTPFP HNSSIDPHGL YSLSLLHTYD QYGDLNFAGM QNTTVLTEKP LSGTDVQEKT FGESQDLPLK SDLGTREDSS VASSDRSSVE REVAEHLAKG FWSDICSTDT PCQMQLSPAV AKDGSEQISQ KRSECPWLGI RISESPEPGQ RTFTTLSSVN CPFISTLSTE GCSSNLEIGN DDYVSEPQQE PCPYACVISL GDDSETDTEG DSESCSAREQ ECEVKLPFNA QRIISLSRND FQSLLKMHKL TPEQLDCIHD IRRRSKNRIA AQRCRKRKLD CIQNLESEIE KLQSEKESLL KERDHILSTL GETKQNLTGL CQKVCKEAAL SQEQIQILAK YSAADCPLSF LISEKDKSTP DGELALPSIF SLSDRPPAVL PPCARGNSEP GYARGQESQQ MSTATSEQAG PAEQCRQSGG ISDFCQQMTD KCTTDE |
| 分子量 | 81 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人BACH蛋白的3篇参考文献概览(均为虚拟示例，实际文献需通过数据库核实)：
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1. **文献名称**: *"Recombinant human BACH1 protein regulates oxidative stress response in mammalian cells"*
**作者**: Tanaka, K. et al.
**摘要**: 研究报道了重组人BACH1蛋白在HeLa细胞中调控抗氧化基因(如HO-1)的机制，证明其通过结合ARE序列抑制氧化应激相关通路，为BACH1在癌症中的作用提供了依据。
2. **文献名称**: *"Structural characterization of BACH2 BTB domain and its interaction with small molecules"*
**作者**: Smith, J.R. et al.
**摘要**: 利用重组表达的人BACH2蛋白BTB结构域进行晶体结构解析，发现其与MAFK转录因子的相互作用，并筛选出可能调控BACH2功能的小分子抑制剂。
3. **文献名称**: *"Recombinant BACH1 facilitates DNA repair by modulating BRCA1-associated pathways"*
**作者**: Lee, S. et al.
**摘要**: 通过纯化的重组BACH1蛋白进行体外实验，证明其与BRCA1协同参与DNA双链断裂修复，揭示了其在维持基因组稳定性中的新功能。
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**备注**：以上文献为示例，实际研究中建议通过PubMed或Google Scholar以关键词**"recombinant BACH1/2 protein"**或**"human BACH transcription factor"**检索近期论文，结合具体研究领域筛选文献。
**Background of Recombinant Human BACH Protein**
Recombinant human BACH (BTB and CNC homology) proteins, such as BACH1 and BACH2. belong to the CNC-bZIP family of transcription factors, characterized by their conserved BTB domain and basic leucine zipper (bZIP) motifs. These proteins play pivotal roles in regulating oxidative stress responses, cellular homeostasis, and immune system modulation. BACH1. for instance, acts as a heme-sensitive repressor of antioxidant genes like *HMOX1* (encoding heme oxygenase-1) by competing with NRF2 for binding to antioxidant response elements (AREs). In contrast, BACH2 is critical for immune cell development, particularly in B-cell maturation and T-cell differentiation, by controlling gene networks involved in antibody class-switching and regulatory T-cell function.
The production of recombinant BACH proteins leverages *in vitro* expression systems (e.g., bacterial, insect, or mammalian cells) to generate purified, functional proteins for research. These recombinant tools enable studies on DNA-protein interactions, transcriptional regulation, and signaling pathways. For example, they are used to dissect how BACH proteins coordinate stress adaptation or immune tolerance, with implications in diseases such as cancer, autoimmune disorders, and neurodegenerative conditions. Structural analyses of recombinant BACH proteins have further clarified their dimerization mechanisms, DNA-binding specificity, and interactions with partners like small Maf proteins.
Overall, recombinant BACH proteins serve as essential reagents for unraveling their dual roles in cellular stress management and immune regulation, offering potential therapeutic targets for modulating antioxidant or immune pathways.
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