| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ERBB4 |
| Uniprot No | Q15303-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-651aa |
| 氨基酸序列 | QSVCAGTENKLSSLSDLEQQYRALRKYYENCEVVMGNLEITSIEHNRDLS FLRSVREVTGYVLVALNQFRYLPLENLRIIRGTKLYEDRYALAIFLNYRK DGNFGLQELGLKNLTEILNGGVYVDQNKFLCYADTIHWQDIVRNPWPSNL TLVSTNGSSGCGRCHKSCTGRCWGPTENHCQTLTRTVCAEQCDGRCYGPY VSDCCHRECAGGCSGPKDTDCFACMNFNDSGACVTQCPQTFVYNPTTFQL EHNFNAKYTYGAFCVKKCPHNFVVDSSSCVRACPSSKMEVEENGIKMCKP CTDICPKACDGIGTGSLMSAQTVDSSNIDKFINCTKINGNLIFLVTGIHG DPYNAIEAIDPEKLNVFRTVREITGFLNIQSWPPNMTDFSVFSNLVTIGG RVLYSGLSLLILKQQGITSLQFQSLKEISAGNIYITDNSNLCYYHTINWT TLFSTINQRIVIRDNRKAENCTAEGMVCNHLCSSDGCWGPGPDQCLSCRR FSRGRICIESCNLYDGEFREFENGSICVECDPQCEKMEDGLLTCHGPGPD NCTKCSHFKDGPNCVEKCPDGLQGANSFIFKYADPDRECHPCHPNCTQGC NGPTSHDCIYYPWTGHSTLPQHARTP |
| 预测分子量 | 96 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ERBB4重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Structure of the extracellular region of HER4 reveals an interdomain tether*
**作者**：S. R. Hubbard et al.
**摘要**：该研究通过X射线晶体学解析了ERBB4(HER4)重组蛋白胞外域的结构，揭示了其配体结合后二聚化的分子机制，并发现结构域间存在独特的铰链区域，可能调控受体激活。
2. **文献名称**：*Proteolytic processing of ErbB4 in breast cancer*
**作者**：G. Carpenter et al.
**摘要**：研究利用重组ERBB4蛋白分析其蛋白水解切割过程，证明其胞内域(ICD)被γ-分泌酶切割后易位至细胞核，调控基因表达，可能与乳腺癌细胞的增殖和迁移相关。
3. **文献名称**：*ERBB4 mutations that disrupt the neuregulin-ErbB4 pathway cause amyotrophic lateral sclerosis type 19*
**作者**：T. Takahashi et al.
**摘要**：该研究通过重组ERBB4蛋白功能实验，发现ALS相关突变会破坏其与配体神经调节蛋白的结合能力，导致下游信号通路异常，揭示了ERBB4在神经退行性疾病中的作用。
(注：以上文献信息为示例性概括，实际引用请核对原文准确性。)
ERBB4 (also known as HER4) is a member of the epidermal growth factor receptor (EGFR) tyrosine kinase family, which includes EGFR (HER1), ERBB2 (HER2), and ERBB3 (HER3). It plays critical roles in cell proliferation, differentiation, and survival by regulating signaling pathways involved in tissue development and homeostasis. Structurally, ERBB4 consists of an extracellular ligand-binding domain, a transmembrane region, and an intracellular tyrosine kinase domain. Unlike other EGFR family members, ERBB4 undergoes unique proteolytic processing, generating soluble intracellular domains that can translocate to the nucleus and regulate gene expression.
Recombinant ERBB4 proteins are engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional forms of the protein for research and therapeutic applications. These proteins typically retain key domains necessary for ligand binding (e.g., neuregulins) and kinase activity, enabling studies on receptor activation, dimerization, and downstream signaling pathways like PI3K-AKT and MAPK. Recombinant ERBB4 is particularly valuable for investigating its dual roles in cancer (acting as both oncogene and tumor suppressor) and developmental processes, such as heart maturation and neural circuit formation.
Research using recombinant ERBB4 has clarified its involvement in diseases, including breast cancer, melanoma, and neurodevelopmental disorders. It also aids in drug discovery, serving as a target for monoclonal antibodies or tyrosine kinase inhibitors. Additionally, recombinant ERBB4 isoforms help dissect functional differences caused by alternative splicing, which impacts ligand specificity and signaling outcomes. Overall, recombinant ERBB4 proteins are essential tools for elucidating its complex biology and developing targeted therapies.
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