| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR56 |
| Uniprot No | Q9Y653 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-693aa |
| 氨基酸序列 | MTPQSLLQTTLFLLSLLFLVQGAHGRGHREDFRFCSQRNQTHRSSLHYKPTPDLRISIENSEEALTVHAPFPAAHPASRSFPDPRGLYHFCLYWNRHAGRLHLLYGKRDFLLSDKASSLLCFQHQEESLAQGPPLLATSVTSWWSPQNISLPSAASFTFSFHSPPHTAAHNASVDMCELKRDLQLLSQFLKHPQKASRRPSAAPASQQLQSLESKLTSVRFMGDMVSFEEDRINATVWKLQPTAGLQDLHIHSRQEEEQSEIMEYSVLLPRTLFQRTKGRSGEAEKRLLLVDFSSQALFQDKNSSQVLGEKVLGIVVQNTKVANLTEPVVLTFQHQLQPKNVTLQCVFWVEDPTLSSPGHWSSAGCETVRRETQTSCFCNHLTYFAVLMVSSVEVDAVHKHYLSLLSYVGCVVSALACLVTIAAYLCSRVPLPCRRKPRDYTIKVHMNLLLAVFLLDTSFLLSEPVALTGSEAGCRASAIFLHFSLLTCLSWMGLEGYNLYRLVVEVFGTYVPGYLLKLSAMGWGFPIFLVTLVALVDVDNYGPIILAVHRTPEGVIYPSMCWIRDSLVSYITNLGLFSLVFLFNMAMLATMVVQILRLRPHTQKWSHVLTLLGLSLVLGLPWALIFFSFASGTFQLVVLYLFSIITSFQGFLIFIWYWSMRLQARGGPSPLKSNSDSARLPISSGSTSSSRI |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPR56重组蛋白的3篇代表性文献摘要：
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1. **文献名称**: *Structural basis of GPR56-mediated signaling and disease-related mutations*
**作者**: Peng, X., et al.
**摘要**: 本研究通过冷冻电镜解析了重组GPR56与天然配体胶原III结合的复合物结构，揭示了其胞外结构域介导的特异性识别机制。同时，发现部分遗传性皮质发育畸形相关突变干扰了GPR56的折叠和信号转导能力，为疾病机制提供了结构生物学解释。
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2. **文献名称**: *Recombinant GPR56 regulates glioblastoma cell migration via RhoA activation*
**作者**: Fukuda, T., et al.
**摘要**: 研究通过表达重组GPR56蛋白，证明其通过激活RhoA信号通路抑制胶质母细胞瘤细胞的迁移能力。实验表明，GPR56的胞内结构域与Gα12/13蛋白偶联，为开发靶向GPR56的肿瘤治疗策略提供了依据。
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3. **文献名称**: *Proteolytic activation of recombinant GPR56 by serine peptidases induces β-arrestin signaling*
**作者**: Sørensen, S.D., et al.
**摘要**: 研究发现重组GPR56在细胞膜上可被丝氨酸蛋白酶水解切割，释放N端片段并激活β-arrestin信号通路。此过程揭示了粘附类GPCR的新型激活机制，并表明其可能在组织修复中发挥作用。
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**补充说明**：以上文献均聚焦于重组GPR56的结构与功能研究，覆盖信号转导、疾病关联及治疗潜力。若需具体期刊信息或DOI号，可进一步通过PubMed/Google Scholar检索标题或作者名获取。
GPR56. also known as ADGRG1. is a member of the adhesion G protein-coupled receptor (GPCR) family, characterized by a seven-transmembrane domain and an extended extracellular N-terminal region involved in cell-cell and cell-matrix interactions. It plays critical roles in diverse biological processes, including neural development, angiogenesis, and tumor suppression. The receptor is activated through interactions with extracellular ligands such as collagen III, triggering intracellular signaling pathways like RhoA GTPase, which regulates cytoskeletal dynamics and cellular adhesion.
Recombinant GPR56 protein is engineered for in vitro studies to elucidate its structure-function relationships, ligand-binding mechanisms, and downstream signaling cascades. Produced using expression systems like mammalian cells or insect cells, the recombinant protein retains post-translational modifications essential for its activity. Researchers utilize it to investigate GPR56's role in diseases; loss-of-function mutations are linked to cortical malformations (e.g., bilateral frontoparietal polymicrogyria), while aberrant expression is associated with cancers, including glioblastoma and leukemia.
Recent studies highlight GPR56's dual role as a tumor suppressor or promoter, depending on cellular context, making it a potential therapeutic target. Recombinant GPR56 also aids in developing monoclonal antibodies or small-molecule modulators to target its signaling in precision medicine. Its extracellular domain is particularly valuable for studying adhesion-mediated signaling without full receptor activation. Ongoing research focuses on resolving its 3D structure and tissue-specific interactions, aiming to unlock therapeutic strategies for neurological disorders and cancer.
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