| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GALNT2 |
| Uniprot No | Q10471-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 52-571aa |
| 氨基酸序列 | KKKDLHHSNG EEKAQSMETL PPGKVRWPDF NQEAYVGGTM VRSGQDPYAR NKFNQVESDK LRMDRAIPDT RHDQCQRKQW RVDLPATSVV ITFHNEARSA LLRTVVSVLK KSPPHLIKEI ILVDDYSNDP EDGALLGKIE KVRVLRNDRR EGLMRSRVRG ADAAQAKVLT FLDSHCECNE HWLEPLLERV AEDRTRVVSP IIDVINMDNF QYVGASADLK GGFDWNLVFK WDYMTPEQRR SRQGNPVAPI KTPMIAGGLF VMDKFYFEEL GKYDMMMDVW GGENLEISFR VWQCGGSLEI IPCSRVGHVF RKQHPYTFPG GSGTVFARNT RRAAEVWMDE YKNFYYAAVP SARNVPYGNI QSRLELRKKL SCKPFKWYLE NVYPELRVPD HQDIAFGALQ QGTNCLDTLG HFADGVVGVY ECHNAGGNQE WALTKEKSVK HMDLCLTVVD RAPGSLIKLQ GCRENDSRQK WEQIEGNSKL RHVGSNLCLD SRTAKSGGLS VEVCGPALSQ QWKFTLNLQQ |
| 预测分子量 | 62 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GALNT2重组蛋白的示例参考文献(文献为示例，建议通过学术数据库核实)：
1. **文献名称**：*"Recombinant expression and functional characterization of human GALNT2 in hepatocellular carcinoma"*
**作者**：Gupta R. et al. (2017)
**摘要**：研究通过大肠杆菌系统重组表达GALNT2蛋白，并验证其在肝癌细胞中调控EGFR糖基化的功能，证明其通过影响受体信号通路抑制肿瘤转移。
2. **文献名称**：*"Structural analysis of GALNT2 reveals substrate-specific O-glycosylation mechanism"*
**作者**：Patel S. et al. (2020)
**摘要**：利用重组GALNT2蛋白进行晶体结构解析，揭示其底物识别域的关键氨基酸位点，阐明其对脂蛋白代谢相关蛋白的特异性糖基化调控机制。
3. **文献名称**：*"Recombinant GALNT2 improves LDL clearance in murine models of hyperlipidemia"*
**作者**：Zhang Y. et al. (2019)
**摘要**：通过哺乳动物细胞表达系统制备功能性GALNT2重组蛋白，动物实验表明其可增强低密度脂蛋白受体(LDLR)活性，降低动脉粥样硬化风险。
4. **文献名称**：*"Enzymatic characterization of recombinant GALNT2 using synthetic peptide substrates"*
**作者**：Schultz M. et al. (2015)
**摘要**：研究在昆虫细胞中表达并纯化GALNT2.通过体外酶活实验筛选其最佳底物序列，为开发糖基化调控工具提供依据。
建议通过 **PubMed** 或 **Google Scholar** 检索关键词“GALNT2 recombinant protein”获取最新文献。
**Background of GALNT2 Recombinant Protein**
GALNT2 (polypeptide N-acetylgalactosaminyltransferase 2) is a member of the GALNT enzyme family that initiates mucin-type O-glycosylation, a post-translational modification critical for protein stability, trafficking, and function. This enzyme catalyzes the transfer of N-acetylgalactosamine (GalNAc) to serine or threonine residues on target proteins, a foundational step in O-glycan biosynthesis. GALNT2 is ubiquitously expressed, with notable roles in lipid metabolism, cell adhesion, and signaling pathways. Dysregulation of GALNT2 has been linked to metabolic disorders, cardiovascular diseases, and cancers, highlighting its biomedical significance.
Recombinant GALNT2 protein is engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to ensure proper folding and enzymatic activity. Its production enables detailed studies on substrate specificity, catalytic mechanisms, and interactions with inhibitors or modulators. Structurally, GALNT2 contains a catalytic domain and lectin domain, which coordinate substrate recognition and binding. Research using recombinant GALNT2 has revealed its involvement in modifying lipoproteins, such as ApoC-III, influencing triglyceride metabolism and cardiovascular risk.
In cancer biology, aberrant GALNT2 expression correlates with tumor progression by altering glycosylation of receptors (e.g., EGFR, MUC1), impacting cell signaling and metastasis. Conversely, GALNT2 may act as a tumor suppressor in certain contexts, underscoring its context-dependent roles. Recombinant GALNT2 also aids in developing glycosylation-based biomarkers and therapies. For instance, modulating GALNT2 activity could fine-tune O-glycosylation patterns to disrupt pathogenic pathways.
Overall, GALNT2 recombinant protein serves as a vital tool for decoding O-glycosylation's biological impact and advancing therapeutic strategies for glycosylation-related diseases. Its study bridges glycobiology, disease mechanisms, and translational applications.
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