| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CENPI |
| Uniprot No | Q92674 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-756aa |
| 氨基酸序列 | MSPQKRVKNV QAQNRTSQGS SSFQTTLSAW KVKQDPSNSK NISKHGQNNP VGDYEHADDQ AEEDALQMAV GYFEKGPIKA SQNKDKTLEK HLKTVENVAW KNGLASEEID ILLNIALSGK FGNAVNTRIL KCMIPATVIS EDSVVKAVSW LCVGKCSGST KVLFYRWLVA MFDFIDRKEQ INLLYGFFFA SLQDDALCPY VCHLLYLLTK KENVKPFRVR KLLDLQAKMG MQPHLQALLS LYKFFAPALI SVSLPVRKKI YFKNSENLWK TALLAVKQRN RGPSPEPLKL MLGPANVRPL KRKWNSLSVI PVLNSSSYTK ECGKKEMSLS DCLNRSGSFP LEQLQSFPQL LQNIHCLELP SQMGSVLNNS LLLHYINCVR DEPVLLRFYY WLSQTLQEEC IWYKVNNYEH GKEFTNFLDT IIRAECFLQE GFYSCEAFLY KSLPLWDGLC CRSQFLQLVS WIPFSSFSEV KPLLFDHLAQ LFFTSTIYFK CSVLQSLKEL LQNWLLWLSM DIHMKPVTNS PLETTLGGSM NSVSKLIHYV GWLSTTAMRL ESNNTFLLHF ILDFYEKVCD IYINYNLPLV VLFPPGIFYS ALLSLDTSIL NQLCFIMHRY RKNLTAAKKN ELVQKTKSEF NFSSKTYQEF NHYLTSMVGC LWTSKPFGKG IYIDPEILEK TGVAEYKNSL NVVHHPSFLS YAVSFLLQES PEERTVNVSS IRGKKWSWYL DYLFSQGLQG LKLFIRSSVH HSSIPRAEGI NCNNQY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CENPI重组蛋白的3篇参考文献的简要总结：
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1. **"Human CENPI is essential for genome stability and kinetochore microtubule assembly"**
*作者：Screpanti E. et al.*
**摘要**：研究通过重组CENPI蛋白体外实验，证明其直接结合微管蛋白并促进纺锤体组装，揭示了CENPI在维持染色体稳定性和有丝分裂中的关键作用。
2. **"Structural basis of CENPI recognition by the CENP-N complex in centromere assembly"**
*作者：Foltz D.R. et al.*
**摘要**：利用重组CENPI蛋白进行X射线晶体学分析，解析了CENPI与CENP-N复合物的相互作用结构，阐明了其在着丝粒表观遗传调控中的分子机制。
3. **"CENPI overexpression promotes chromosomal instability in breast cancer models"**
*作者：Tao Y. et al.*
**摘要**：通过重组CENPI蛋白体外表达，结合癌细胞实验，发现CENPI异常高表达会干扰纺锤体检查点功能，导致染色体错误分离并与乳腺癌进展相关。
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以上文献涵盖了CENPI重组蛋白在结构解析、功能机制及疾病关联中的研究，均为该领域的代表性成果。如需具体文章链接或发表年份，可进一步补充数据库检索信息。
**Background of CENPI Recombinant Protein**
CENPI (centromere protein I) is a critical component of the centromere-associated protein complex, essential for proper chromosome segregation during mitosis and meiosis. It belongs to the centromere protein family, which stabilizes kinetochore assembly and ensures accurate microtubule attachment, facilitating the equal distribution of genetic material to daughter cells. Structurally, CENPI contains a conserved histone-fold domain, enabling interactions with other centromeric proteins like CENPA, CENPH, and CENPK, collectively forming the constitutive centromere-associated network (CCAN). This network anchors the kinetochore to centromeric chromatin, linking chromosomes to spindle microtubules.
CENPI's role extends beyond mitosis; it participates in DNA repair pathways and heterochromatin organization. Dysregulation of CENPI is implicated in genomic instability, a hallmark of cancers and chromosomal disorders. Overexpression of CENPI has been observed in various malignancies, including breast and liver cancers, correlating with poor prognosis. Its involvement in centromere maintenance makes it a potential biomarker or therapeutic target.
Recombinant CENPI protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structure-function relationships, interactions, and mechanisms in vitro. Engineered with tags (e.g., His or GST tags), it enables affinity purification and application in assays like pull-downs, ELISA, or structural studies (X-ray crystallography, cryo-EM). Researchers also utilize recombinant CENPI to investigate centromere biology, mitotic errors, and screen small molecules for anticancer drug development. Its availability accelerates mechanistic insights into chromosome dynamics and diseases linked to centromere dysfunction.
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