| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CENPF |
| Uniprot No | P49454 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1759-2093aa |
| 氨基酸序列 | AIQGRNESCDISKEHTSETTERTPKHDVHQICDKDAQQDLNLDIEKITETGAVKPTGECSGEQSPDTNYEPPGEDKTQGSSECISELSFSGPNALVPMDFLGNQEDIHNLQLRVKETSNENLRLLHVIEDRDRKVESLLNEMKELDSKLHLQEVQLMTKIEACIELEKIVGELKKENSDLSEKLEYFSCDHQELLQRVETSEGLNSDLEMHADKSSREDIGDNVAKVNDSWKERFLDVENELSRIRSEKASIEHEALYLEADLEVVQTEKLCLEKDNENKQKVIVCLEEELSVVTSERNQLRGELDTMSKKTTALDQLSEKMKEKTQELESHQSE |
| 预测分子量 | 82.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CENPF重组蛋白的3篇代表性文献及其摘要内容的简要概述：
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1. **文献名称**：*CENPF regulates G1/S transition and metastatic potential in hepatocellular carcinoma*
**作者**：Liao, H., et al.
**摘要**：研究通过构建CENPF重组蛋白，验证其在肝癌细胞周期G1/S期转换中的调控作用，并发现其过表达促进肿瘤转移，为肝癌治疗提供潜在靶点。
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2. **文献名称**：*Centromere Protein F interacts with BRCA1 to regulate DNA repair*
**作者**：Varis, A., et al.
**摘要**：利用CENPF重组蛋白进行互作实验，发现其与BRCA1结合并参与DNA损伤修复，提示其在基因组稳定性维持中的新功能。
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3. **文献名称**：*Recombinant CENPF as a biomarker for aggressive prostate cancer*
**作者**：Sun, J., et al.
**摘要**：通过重组CENPF蛋白开发特异性抗体，证实其在晚期前列腺癌患者中高表达，可作为预后不良的生物标志物。
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注：上述文献为示例性质，实际文献需通过数据库(如PubMed)检索确认。建议结合关键词“CENPF recombinant protein”或“CENPF overexpression”进一步查找。
CENPF (Centromere Protein F), also known as mitosin, is a large nuclear protein crucial for cell division, particularly during mitosis and meiosis. It plays essential roles in chromosome segregation, kinetochore assembly, and cell cycle regulation. Structurally, CENPF contains multiple functional domains, including an N-terminal region involved in microtubule binding and a C-terminal domain that interacts with other centromere components. Its expression peaks during the G2/M phase of the cell cycle, correlating with its mitotic functions.
Recombinant CENPF protein is engineered using molecular cloning techniques, typically expressed in mammalian or bacterial systems with affinity tags (e.g., His-tag) for purification. This biotechnologically produced protein retains native structural and functional properties, enabling in vitro studies of its interactions with chromosomal DNA, microtubules, and regulatory partners like CENP-E and Aurora kinases.
Research applications include investigating mechanisms of aneuploidy, cancer biology (CENPF overexpression is linked to poor prognosis in multiple cancers), and congenital disorders related to mitotic errors. Recombinant CENPF also serves as an antigen for antibody development and a tool for drug screening targeting cell cycle dysregulation. Its ~350 kDa size and dynamic phosphorylation patterns (regulated by CDK1/cyclin B1) make it a valuable model for studying post-translational modifications in protein function. Current studies focus on its non-mitotic roles in nuclear envelope reassembly and transcriptional regulation, expanding its therapeutic relevance in proliferative diseases.
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