| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AMY2A |
| Uniprot No | P04746 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 16-511aa |
| 氨基酸序列 | QYSPN TQQGRTSIVH LFEWRWVDIA LECERYLAPK GFGGVQVSPP NENVAIYNPF RPWWERYQPV SYKLCTRSGN EDEFRNMVTR CNNVGVRIYV DAVINHMCGN AVSAGTSSTC GSYFNPGSRD FPAVPYSGWD FNDGKCKTGS GDIENYNDAT QVRDCRLTGL LDLALEKDYV RSKIAEYMNH LIDIGVAGFR LDASKHMWPG DIKAILDKLH NLNSNWFPAG SKPFIYQEVI DLGGEPIKSS DYFGNGRVTE FKYGAKLGTV IRKWNGEKMS YLKNWGEGWG FVPSDRALVF VDNHDNQRGH GAGGASILTF WDARLYKMAV GFMLAHPYGF TRVMSSYRWP RQFQNGNDVN DWVGPPNNNG VIKEVTINPD TTCGNDWVCE HRWRQIRNMV IFRNVVDGQP FTNWYDNGSN QVAFGRGNRG FIVFNNDDWS FSLTLQTGLP AGTYCDVISG DKINGNCTGI KIYVSDDGKA HFSISNSAED PFIAIHAESK L |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AMY2A重组蛋白的3篇参考文献示例：
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1. **文献名称**：*Expression and characterization of recombinant human pancreatic α-amylase (AMY2A) in Pichia pastoris*
**作者**：Zhang Y, et al.
**摘要**：本研究利用毕赤酵母系统高效表达人源AMY2A重组蛋白，通过优化培养条件提高产量，并验证其酶活性与天然蛋白相似，适用于工业淀粉水解应用。
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2. **文献名称**：*Crystal structure of AMY2A reveals insights into substrate specificity and calcium-dependent activity*
**作者**：Suzuki K, et al.
**摘要**：解析了重组AMY2A的晶体结构，发现其底物结合位点具有独特的空间构象，且钙离子对其催化活性至关重要，为酶工程改造提供结构基础。
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3. **文献名称**：*Functional analysis of AMY2A mutants in starch digestion using recombinant protein assays*
**作者**：Wang L, et al.
**摘要**：通过定点突变技术构建多个AMY2A突变体，重组表达后检测其酶动力学参数，揭示了关键氨基酸残基对淀粉水解效率的影响机制。
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(注：上述文献为示例，实际研究中建议通过PubMed或Web of Science检索具体论文。)
**Background of AMY2A Recombinant Protein**
AMY2A (Alpha-Amylase 2A) is a glycoside hydrolase enzyme encoded by the *AMY2A* gene in humans. It belongs to the α-amylase family, which catalyzes the hydrolysis of α-1.4-glycosidic bonds in starch and related polysaccharides, breaking them into smaller sugars like maltose and glucose. AMY2A is primarily expressed in the pancreas and secreted into the small intestine, where it plays a critical role in carbohydrate digestion. Unlike AMY1A (salivary amylase), which initiates starch digestion in the mouth, AMY2A functions in the duodenum, complementing the digestive process.
Structurally, AMY2A is a single-chain protein (~55 kDa) with a conserved catalytic domain featuring a (β/α)₈ barrel fold. It requires calcium ions for stability and chloride ions as a cofactor for optimal activity. The enzyme’s specificity for starch substrates and its endo-hydrolase activity make it essential for efficient nutrient absorption. Dysregulation of AMY2A has been linked to pancreatic disorders, such as pancreatitis, and elevated serum levels of amylase (including AMY2A) serve as diagnostic markers for pancreatic damage.
Recombinant AMY2A is produced using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*), enabling high-purity, scalable production for research and industrial applications. Its recombinant form retains native enzymatic properties, allowing studies on substrate specificity, inhibitor screening, and enzyme kinetics. In biotechnology, AMY2A is utilized in starch-processing industries (e.g., food, biofuels) and as a tool to develop enzyme-based therapies or diagnostic assays. Research also explores its role in metabolic diseases, such as diabetes, due to its impact on carbohydrate metabolism.
Overall, AMY2A recombinant protein bridges fundamental biochemistry with practical applications, underscoring its importance in both physiological and industrial contexts.
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