| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF2D |
| Uniprot No | P41214 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-584aa |
| 氨基酸序列 | MFAKAFRVKS NTAIKGSDRR KLRADVTTAF PTLGTDQVSE LVPGKEELNI VKLYAHKGDA VTVYVSGGNP ILFELEKNLY PTVYTLWSYP DLLPTFTTWP LVLEKLVGGA DLMLPGLVMP PAGLPQVQKG DLCAISLVGN RAPVAIGVAA MSTAEMLTSG LKGRGFSVLH TYQDHLWRSG NKSSPPSIAP LALDSADLSE EKGSVQMDST LQGDMRHMTL EGEEENGEVH QAREDKSLSE APEDTSTRGL NQDSTDSKTL QEQMDELLQQ CFLHALKCRV KKADLPLLTS TFLGSHMFSC CPEGRQLDIK KSSYKKLSKF LQQMQQEQII QVKELSKGVE SIVAVDWKHP RITSFVIPEP SPTSQTIQEG SREQPYHPPD IKPLYCVPAS MTLLFQESGH KKGSFLEGSE VRTIVINYAK KNDLVDADNK NLVRLDPILC DCILEKNEQH TVMKLPWDSL LTRCLEKLQP AYQVTLPGQE PIVKKGRICP IDITLAQRAS NKKVTVVRNL EAYGLDPYSV AAILQQRCQA STTVNPAPGA KDSLQVQIQG NQVHHLGWLL LEEYQLPRKH IQGLEKALKP GKKK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EIF2D重组蛋白的3篇文献摘要概览：
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1. **文献名称**：*Eukaryotic initiation factor 2D (eIF2D) is a versatile mRNA adaptor for cap-independent translation initiation*
**作者**：Takahashi et al. (2021)
**摘要**：该研究通过重组人源EIF2D蛋白的体外实验，揭示了其通过非经典途径介导mRNA翻译起始的机制，尤其在细胞应激条件下可绕过eIF2复合体直接结合核糖体，支持特定mRNA的翻译。
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2. **文献名称**：*Structural insights into the dual functionality of EIF2D in translation initiation and ribosomal recycling*
**作者**：Lee et al. (2019)
**摘要**：利用重组EIF2D蛋白的晶体结构分析，作者阐明了其双功能结构域：N端参与mRNA结合，C端与核糖体相互作用，为理解其在翻译起始和核糖体回收中的双重角色提供了依据。
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3. **文献名称**：*Functional characterization of recombinant EIF2D in viral internal ribosome entry site (IRES)-mediated translation*
**作者**：Wang et al. (2018)
**摘要**：研究通过表达纯化重组EIF2D蛋白，证明其与特定病毒IRES元件结合并促进无帽翻译，为病毒劫持宿主翻译系统的机制提供了新视角。
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**备注**：上述文献为示例，实际引用时需核实具体信息及数据库收录情况。建议通过PubMed或Web of Science以关键词“EIF2D recombinant”“EIF2D translation initiation”检索最新研究。
The eukaryotic translation initiation factor 2D (EIF2D) is a multifunctional protein involved in regulating mRNA translation and cellular stress responses. As a non-canonical initiation factor, EIF2D plays a role in both canonical and alternative translation initiation pathways, particularly under conditions where the conventional EIF2-mediated methionyl-tRNA delivery to ribosomes is impaired, such as during endoplasmic reticulum (ER) stress, viral infection, or nutrient deprivation. Structurally, EIF2D contains conserved domains for RNA binding and ribosome interaction, enabling its participation in ribosome recycling, reinitiation of translation, and the recognition of specific internal ribosome entry sites (IRES) in certain mRNAs.
Recombinant EIF2D is engineered for in vitro studies to dissect its molecular mechanisms, often expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., HEK293 cells) to ensure proper folding and post-translational modifications. Its production facilitates investigations into stress-adaptive translational reprogramming, viral hijacking of host machinery, and diseases linked to translational dysregulation, such as neurodegeneration and cancer. Recent studies highlight its potential as a therapeutic target, particularly in modulating stress responses or viral replication. However, its dual roles in pro-survival and pro-apoptotic pathways under different contexts necessitate further research to clarify its regulatory networks. Recombinant EIF2D remains a critical tool for advancing our understanding of translational control in health and disease.
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