| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SELE |
| Uniprot No | P16581 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-610aa |
| 氨基酸序列 | MIASQFLSALTLVLLIKESGAWSYNTSTEAMTYDEASAYCQQRYTHLVAIQNKEEIEYLNSILSYSPSYYWIGIRKVNNVWVWVGTQKPLTEEAKNWAPGEPNNRQKDEDCVEIYIKREKDVGMWNDERCSKKKLALCYTAACTNTSCSGHGECVETINNYTCKCDPGFSGLKCEQIVNCTALESPEHGSLVCSHPLGNFSYNSSCSISCDRGYLPSSMETMQCMSSGEWSAPIPACNVVECDAVTNPANGFVECFQNPGSFPWNTTCTFDCEEGFELMGAQSLQCTSSGNWDNEKPTCKAVTCRAVRQPQNGSVRCSHSPAGEFTFKSSCNFTCEEGFMLQGPAQVECTTQGQWTQQIPVCEAFQCTALSNPERGYMNCLPSASGSFRYGSSCEFSCEQGFVLKGSKRLQCGPTGEWDNEKPTCEAVRCDAVHQPPKGLVRCAHSPIGEFTYKSSCAFSCEEGFELHGSTQLECTSQGQWTEEVPSCQVVKCSSLAVPGKINMSCSGEPVFGTVCKFACPEGWTLNGSAARTCGATGHWSGLLPTCEAPTESNIPLVAGLSAAGLSLLTLAPFLLWLRKCLRKAKKFVPASSCQSLESDGSYQKPSYIL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与SELE(E-selectin)重组蛋白相关的参考文献示例(内容为虚构概括，仅供参考)：
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1. **文献名称**: *"High-yield Expression and Purification of Recombinant Human E-selectin in Mammalian Cells"*
**作者**: Smith A, et al.
**摘要**: 该研究描述了一种在HEK293细胞中高效表达重组人E-selectin蛋白的方法，通过优化糖基化修饰和纯化流程，获得了具有生物活性的可溶性SELE蛋白，可用于体外白细胞黏附实验。
2. **文献名称**: *"Structural Analysis of Recombinant E-selectin/Ligand Interactions by Surface Plasmon Resonance"*
**作者**: Chen L, et al.
**摘要**: 利用表面等离子体共振技术(SPR)分析重组E-selectin蛋白与sLeX(sialyl Lewis X)配体的结合动力学，揭示了关键氨基酸残基对亲和力的影响，为抗炎药物设计提供依据。
3. **文献名称**: *"Recombinant E-selectin Fusion Protein Attenuates Neutrophil Infiltration in Murine Sepsis Models"*
**作者**: Wang Y, et al.
**摘要**: 研究构建了SELE-IgG Fc融合蛋白，证明其通过阻断白细胞迁移显著减轻小鼠脓毒症模型的炎症反应，提示重组SELE在治疗炎症性疾病中的潜在应用。
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注：以上文献为示例性内容，实际引用请通过PubMed或Web of Science等数据库检索真实文献。如需具体文章，建议补充关键词或研究方向以便精准查询。
SELE (E-selectin), also known as CD62E or endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein belonging to the selectin family of adhesion molecules. It is primarily expressed by activated endothelial cells in response to inflammatory cytokines such as TNF-α or IL-1β. SELE plays a critical role in mediating the initial tethering and rolling of leukocytes (particularly neutrophils) on vascular endothelium during inflammatory responses, a key step in leukocyte extravasation and immune surveillance.
Structurally, SELE consists of an N-terminal lectin domain, an epidermal growth factor (EGF)-like domain, multiple complement regulatory protein (CRP) repeats, a transmembrane domain, and a short cytoplasmic tail. Its lectin domain specifically binds sialylated carbohydrate ligands like sialyl Lewis X (sLeX) on leukocyte surfaces. This interaction is calcium-dependent and facilitates leukocyte-endothelial adhesion under shear stress conditions.
Recombinant SELE protein is produced using mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications, particularly glycosylation essential for ligand binding. Researchers utilize SELE recombinant proteins in various applications: studying leukocyte trafficking mechanisms, modeling inflammatory diseases, screening anti-inflammatory drugs, and investigating cancer metastasis (as SELE also participates in tumor cell adhesion). Its soluble form (sSELE) is explored as a biomarker in cardiovascular diseases and sepsis. Current therapeutic strategies targeting SELE focus on developing inhibitors to block pathological inflammation while preserving host defense functions.
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