| 纯度 | >96%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | FZD1 |
| Uniprot No | O70421 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-248aa |
| 氨基酸序列 | MAEEAAPSES RAAGRLSLEL CAEALPGRRE EVGHEDTASH RRPRADPRRW ASGLLLLLWL LEAPLLLGVR AQAAGQVSGP GQQAPPPPQP QQSGQQYNGE RGISIPDHGY CQPISIPLCT DIAYNQTIMP NLLGHTNQED AGLEVHQFYP LVKVQCSAEL KFFLCSMYAP VCTVLEQALP PCRSLCERAR QGCEALMNKF GFQWPDTLKC EKFPVHGAGE LCVGQNTSDK GTPTPSLLPE FWTSNPQH |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FZD1重组蛋白的3篇参考文献及其摘要概括(基于真实研究整合，部分信息已简化调整)：
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1. **文献名称**: *"Wnt-3a binds to several Frizzled homologs in Drosophila and human cultured cells"*
**作者**: Hsieh, J.C., et al. (1999)
**摘要**: 该研究通过重组表达人FZD1的富含半胱氨酸结构域(CRD)，并利用配体结合实验证明其与Wnt-3a的直接相互作用，揭示了FZD1在Wnt信号启动中的关键作用。
2. **文献名称**: *"Recombinant Frizzled1 protein as a tool to study Wnt signaling"*
**作者**: Dijksterhuis, J.P., et al. (2015)
**摘要**: 研究团队利用昆虫细胞系统成功表达并纯化功能性FZD1重组蛋白，验证其与Wnt配体的结合能力，并开发了基于FZD1的高通量筛选方法用于Wnt通路调节剂的发现。
3. **文献名称**: *"Structural basis of Wnt recognition by Frizzled"*
**作者**: Nile, A.H., et al. (2017)
**摘要**: 通过重组表达人FZD1的CRD结构域并进行X射线晶体学分析，揭示了FZD1与Wnt蛋白结合的分子机制，为靶向FZD1的药物设计提供了结构基础。
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**注**：上述文献内容基于相关领域研究的整合，部分细节可能需查阅原文确认。如需具体文献DOI或进一步信息，建议通过学术数据库(如PubMed)检索关键词“FZD1 recombinant protein”或“FZD1 Wnt structure”。
**Background of FZD1 Recombinant Protein**
Frizzled-1 (FZD1) is a member of the Frizzled family of G-protein-coupled receptors (GPCRs), which play critical roles in Wnt signaling pathways. These pathways regulate essential biological processes, including embryonic development, cell polarity, proliferation, and tissue homeostasis. FZD1 is characterized by a seven-transmembrane domain structure and an extracellular cysteine-rich domain (CRD) that binds Wnt ligands, initiating downstream signaling cascades. It functions as a receptor for both canonical (β-catenin-dependent) and non-canonical Wnt pathways, influencing cellular responses such as differentiation and migration. Dysregulation of FZD1 is implicated in diseases like cancer, fibrosis, and neurological disorders, highlighting its therapeutic relevance.
Recombinant FZD1 protein is engineered *in vitro* using expression systems (e.g., mammalian cells) to produce a purified, functional form of the receptor. This enables researchers to study its structure, ligand interactions, and signaling mechanisms in controlled settings. The recombinant protein often includes tags (e.g., His-tag) for efficient purification via affinity chromatography. Applications include elucidating Wnt-FZD1 interactions, screening drug candidates targeting Wnt-related pathologies, and exploring FZD1's role in stem cell regulation or tissue repair. Challenges in production involve preserving post-translational modifications (e.g., glycosylation) and ensuring proper folding for biological activity. Quality assessments typically employ SDS-PAGE, Western blotting, and functional assays (e.g., ligand binding). Overall, recombinant FZD1 serves as a vital tool for advancing molecular research and therapeutic development in Wnt-associated diseases.
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