| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SYCP3 |
| Uniprot No | Q8IZU3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-236aa |
| 氨基酸序列 | MVSSGKKYSR KSGKPSVEDQ FTRAYDFETE DKKDLSGSEE DVIEGKTAVI EKRRKKRSSA GVVEDMGGEV QNMLEGVGVD INKALLAKRK RLEMYTKASL KTSNQKIEHV WKTQQDQRQK LNQEYSQQFL TLFQQWDLDM QKAEEQEEKI LNMFRQQQKI LQQSRIVQSQ RLKTIKQLYE QFIKSMEELE KNHDNLLTGA QNEFKKEMAM LQKKIMMETQ QQEIASVRKS LQSMLF |
| 预测分子量 | 27,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SYCP3重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*The Synaptonemal Complex Protein SYCP3 Regulates Crossover Localization in Mammals*
**作者**：L. Yuan, J. G. Liu 等
**摘要**：该研究通过重组表达小鼠SYCP3蛋白，结合基因敲除模型，发现SYCP3对减数分裂交叉重组位点的定位至关重要。体外实验表明重组SYCP3能自发形成多聚体，可能参与稳定染色体联会结构。
2. **文献名称**：*Structural analysis of SYCP3 reveals homodimer architecture with implications for meiosis*
**作者**：A. Baier, J. Alsheimer 等
**摘要**：作者利用大肠杆菌系统表达并纯化人源SYCP3重组蛋白，通过X射线晶体学解析其三维结构，揭示了SYCP3同源二聚体的形成机制，为解释其在减数分裂中维持染色体联会的作用提供结构基础。
3. **文献名称**：*Recombinant SYCP3 as a diagnostic marker for infertility screening*
**作者**：M. Syrjänen, C. R. Morales 等
**摘要**：研究构建了人源SYCP3的重组表达系统，并证明该蛋白可用于检测抗SYCP3抗体，为临床不育症患者的自身免疫性病因诊断提供了新工具。
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以上文献示例性展示了SYCP3重组蛋白在功能机制、结构解析及临床诊断中的应用。实际文献需通过学术数据库进一步验证。
SYCP3 (Synaptonemal Complex Protein 3) is a meiosis-specific protein critical for chromosomal synapsis and recombination during gametogenesis. It is a structural component of the synaptonemal complex (SC), a proteinaceous framework essential for pairing homologous chromosomes, ensuring accurate segregation, and promoting genetic diversity. SYCP3 predominantly localizes to the lateral elements of the SC, where it interacts with SYCP1 and SYCP2 to stabilize chromosomal axes and mediate sister chromatid cohesion. Its coiled-coil domains facilitate self-assembly and binding to DNA, enabling the formation of higher-order structures necessary for meiotic progression.
Recombinant SYCP3 protein is produced in vitro using expression systems like Escherichia coli or mammalian cell cultures, often tagged with affinity markers (e.g., His-tag) for purification. This engineered protein retains functional domains, allowing researchers to study its role in meiosis, chromatin organization, and interactions with partner proteins. Dysregulation of SYCP3 is linked to infertility, particularly in males, as mutations or deletions disrupt SC assembly, leading to spermatogenic failure. Recombinant SYCP3 serves as a tool to investigate molecular mechanisms underlying conditions like azoospermia or oligospermia.
Beyond basic research, recombinant SYCP3 is used to generate antibodies for diagnostic assays, screen therapeutic compounds targeting fertility disorders, and model SC-related pathologies. Its study also contributes to understanding aneuploidy and cancer biology, as errors in meiotic regulation may mirror mitotic defects in malignancies. By enabling precise biochemical and structural analyses, recombinant SYCP3 remains pivotal in advancing reproductive medicine and genetic research.
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