| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACO1 |
| Uniprot No | P21399 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-889aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSNPFAHLAEPLDPVQPGKKFFNLNKL EDSRYGRLPFSIRVLLEAAIRNCDEFLVKKQDIENILHWNVTQHKNIEVP FKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVIDHS IQVDFNRRADSLQKNQDLEFERNRERFEFLKWGSQAFHNMRIIPPGSGII HQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGILGWGVGGIEAE AVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKF VEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSITYLVQTGRDE EKLKYIKKYLQAVGMFRDFNDPSQDPDFTQVVELDLKTVVPCCSGPKRPQ DKVAVSDMKKDFESCLGAKQGFKGFQVAPEHHNDHKTFIYDNTEFTLAHG SVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLNVMPYIKTSLSPGSGV VTYYLQESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLV AVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGV NAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALA TPSDKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTT DHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANI RLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAG SSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADAL GLTGQERYTIIIPENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNG GILNYMIRKMAK |
| 预测分子量 | 101 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACO1重组蛋白的参考文献示例(部分信息基于合理推测，实际文献需进一步验证)：
1. **"Recombinant expression and functional characterization of human ACO1 in Escherichia coli"**
- **作者**: Smith J, et al.
- **摘要**: 研究报道了人源ACO1基因在大肠杆菌中的重组表达与纯化，验证了其酶活性及铁调控功能，为体外研究ACO1的代谢作用提供了工具。
2. **"Structural insights into the dual roles of ACO1 in iron homeostasis and citrate metabolism"**
- **作者**: Tanaka K, et al.
- **摘要**: 通过X射线晶体学解析重组ACO1蛋白结构，揭示了其同时作为乌头酸酶和铁调节蛋白(IRP1)的构象变化机制。
3. **"Iron-dependent regulation of ACO1: Implications for cellular adaptation to oxidative stress"**
- **作者**: Chen L, et al.
- **摘要**: 利用重组ACO1蛋白探究铁离子对其酶活性和RNA结合能力的调控，阐明了ACO1在细胞氧化应激反应中的双重功能。
4. **"ACO1 mutations disrupt cellular metabolism and are linked to hereditary hyperferritinemia"**
- **作者**: Rossi A, et al.
- **摘要**: 通过重组突变体蛋白实验，发现ACO1基因突变导致酶活性丧失，并促进铁蛋白异常积累，为相关疾病的分子机制提供了证据。
**注**：以上文献名为示例，建议通过PubMed或Web of Science以“ACO1 recombinant protein”“ACO1 iron regulation”等关键词检索真实文献。
ACO1 (Aconitase 1) is a multifunctional protein with dual roles in cellular metabolism and iron regulation. As a member of the aconitase family, it catalyzes the stereoisomeric conversion of citrate to isocitrate in the tricarboxylic acid (TCA) cycle, a critical step for ATP production in mitochondria. Beyond its enzymatic function, Aconitase 1 also acts as an iron regulatory protein (IRP1) in the cytoplasm. Under low iron conditions, it binds to iron-responsive elements (IREs) in mRNA to regulate the expression of proteins involved in iron uptake, storage, and utilization, such as ferritin and transferrin receptor. This dual functionality links cellular energy metabolism with iron homeostasis.
Recombinant ACO1 protein is produced through genetic engineering, typically using expression systems like *E. coli* or mammalian cells. Its structure includes four domains arranged around a catalytic [4Fe-4S] cluster, essential for enzymatic activity. Researchers utilize recombinant ACO1 to study mechanisms of mitochondrial dysfunction, oxidative stress (due to iron-sulfur cluster sensitivity), and diseases like iron-deficiency anemia, neurodegenerative disorders, or cancer. It also serves as a tool for exploring post-translational modifications (e.g., oxidation) that switch ACO1 between its enzymatic and RNA-binding roles. Studies often focus on how ACO1 mutations or dysregulation contribute to metabolic syndromes or iron-related pathologies. Its recombinant form enables high-purity, scalable production for biochemical assays, structural studies, and drug discovery targeting metabolic or iron-imbalance diseases.
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