| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOSIP |
| Uniprot No | Q9Y314 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-301aa |
| 氨基酸序列 | MTRHGKNCTA GAVYTYHEKK KDTAASGYGT QNIRLSRDAV KDFDCCCLSL QPCHDPVVTP DGYLYEREAI LEYILHQKKE IARQMKAYEK QRGTRREEQK ELQRAASQDH VRGFLEKESA IVSRPLNPFT AKALSGTSPD DVQPGPSVGP PSKDKDKVLP SFWIPSLTPE AKATKLEKPS RTVTCPMSGK PLRMSDLTPV HFTPLDSSVD RVGLITRSER YVCAVTRDSL SNATPCAVLR PSGAVVTLEC VEKLIRKDMV DPVTGDKLTD RDIIVLQRGG TGFAGSGVKL QAEKSRPVMQ A |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于NOSIP重组蛋白的相关文献摘要概述：
1. **文献名称**: "Characterization of NOSIP as a modulator of eNOS activity in endothelial cells"
**作者**: Dedio J et al.
**摘要**: 研究通过重组表达人源NOSIP蛋白，发现其通过与eNOS直接结合，抑制酶活性并调控内皮细胞一氧化氮生成，揭示了其在血管稳态中的作用机制。
2. **文献名称**: "Recombinant NOSIP interacts with the E3 ubiquitin ligase E6AP and regulates protein stability"
**作者**: Schüller M et al.
**摘要**: 利用重组NOSIP蛋白进行互作实验，发现其与泛素连接酶E6AP结合，参与靶蛋白的泛素化降解通路，提示其在细胞蛋白稳定性调控中的新功能。
3. **文献名称**: "Expression and purification of functional NOSIP in a bacterial system for structural studies"
**作者**: Kölker K et al.
**摘要**: 开发了一种大肠杆菌重组表达系统，成功纯化具有生物活性的NOSIP蛋白，并通过X射线晶体学初步解析其结构，为后续功能研究提供基础。
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注：上述文献为示例性质，实际文献需通过PubMed或Web of Science检索确认。建议以关键词“NOSIP recombinant”“NOSIP expression”在数据库查询最新研究。
NOSIP (Nitric Oxide Synthase Interacting Protein) is a cellular protein initially identified for its role in regulating nitric oxide synthase (NOS) activity, which governs the production of nitric oxide (NO), a critical signaling molecule involved in vascular homeostasis, neurotransmission, and immune responses. Discovered in the early 2000s, NOSIP interacts with endothelial NOS (eNOS) and neuronal NOS (nNOS), influencing their subcellular localization, stability, and enzymatic activity. It acts as a scaffold or adaptor protein, promoting the translocation of NOS isoforms from the plasma membrane to intracellular compartments, thereby modulating NO bioavailability. This regulatory mechanism is vital for maintaining vascular tone, angiogenesis, and neuronal function.
Recombinant NOSIP protein, produced via expression systems like *E. coli* or mammalian cells, enables detailed study of its structure-function relationships and interactions. Its purified form is used in biochemical assays, crystallography, and cell-based experiments to dissect its role in NOS regulation and downstream pathways. Research highlights its involvement in pathologies such as hypertension, atherosclerosis, and neurodegenerative disorders, where dysregulated NO signaling is implicated. Additionally, NOSIP has emerging roles beyond NO regulation, including interactions with transcription factors and participation in cellular stress responses, suggesting broader functional significance.
The development of recombinant NOSIP has advanced drug discovery efforts, particularly in designing molecules to target NO-related pathways. Its application in disease models underscores its therapeutic potential, though challenges remain in understanding tissue-specific effects and optimizing delivery strategies. Overall, recombinant NOSIP serves as a key tool for unraveling the complexities of NO biology and developing interventions for related diseases.
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