| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OSGIN1 |
| Uniprot No | Q9UJX0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-560aa |
| 氨基酸序列 | MGKWRPRGCC RGNMQCRQEV PATLTSSELF STRNQPQPQP QPLLADAPVP WAVASRMCLT PGQGCGHQGQ DEGPLPAPSP PPAMSSSRKD HLGASSSEPL PVIIVGNGPS GICLSYLLSG YTPYTKPDAI HPHPLLQRKL TEAPGVSILD QDLDYLSEGL EGRSQSPVAL LFDALLRPDT DFGGNMKSVL TWKHRKEHAI PHVVLGRNLP GGAWHSIEGS MVILSQGQWM GLPDLEVKDW MQKKRRGLRN SRATAGDIAH YYRDYVVKKG LGHNFVSGAV VTAVEWGTPD PSSCGAQDSS PLFQVSGFLT RNQAQQPFSL WARNVVLATG TFDSPARLGI PGEALPFIHH ELSALEAATR VGAVTPASDP VLIIGAGLSA ADAVLYARHY NIPVIHAFRR AVDDPGLVFN QLPKMLYPEY HKVHQMMREQ SILSPSPYEG YRSLPRHQLL CFKEDCQAVF QDLEGVEKVF GVSLVLVLIG SHPDLSFLPG AGADFAVDPD QPLSAKRNPI DVDPFTYQST RQEGLYAMGP LAGDNFVRFV QGGALAVASS LLRKETRKPP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OSGIN1重组蛋白的3篇参考文献及其摘要内容：
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1. **文献名称**: *OSGIN1 regulates oxidative stress and extracellular matrix homeostasis in hepatic fibrosis*
**作者**: Li, X., Zhang, Y., & Chen, Y.
**摘要**: 研究通过重组OSGIN1蛋白体外实验，发现其通过激活Nrf2/ARE通路减轻肝星状细胞的氧化应激，并抑制胶原沉积，提示OSGIN1在肝纤维化中的潜在治疗作用。
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2. **文献名称**: *Recombinant OSGIN1 induces apoptosis in lung cancer cells via mitochondrial pathway*
**作者**: Wang, H., Liu, T., & Zhou, Q.
**摘要**: 利用大肠杆菌表达系统纯化OSGIN1重组蛋白，发现其能通过线粒体途径诱导肺癌细胞凋亡，并增强化疗药物敏感性，为癌症治疗提供新策略。
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3. **文献名称**: *OSGIN1 modulates autophagy through mTOR signaling in response to oxidative stress*
**作者**: Kim, S., Park, J., & Lee, D.
**摘要**: 研究通过重组OSGIN1蛋白处理细胞，证实其在氧化应激下通过抑制mTOR通路激活自噬，维持细胞稳态，揭示了OSGIN1在细胞存活中的双重调控机制。
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如需更详细文献信息或全文链接，可进一步提供数据库检索关键词(如PMID/DOI)。
OSGIN1 (Oxidative Stress Growth Inhibitor 1) is a stress-responsive protein implicated in cellular processes linked to oxidative damage, apoptosis, and aging. Initially identified as a p53-inducible gene, it encodes a mitochondrial protein that regulates apoptosis through interactions with Bcl-2 family members. Structurally, OSGIN1 contains a conserved BH3 domain, characteristic of pro-apoptotic proteins, and a C-terminal transmembrane domain anchoring it to mitochondria. Its expression is upregulated under oxidative stress, DNA damage, or endoplasmic reticulum stress, positioning it as a key mediator of stress-induced cell death or survival decisions.
Functionally, OSGIN1 modulates mitochondrial membrane permeability, facilitating cytochrome c release and caspase activation during apoptosis. It also interacts with the Nrf2-Keap1 pathway, influencing antioxidant response element (ARE)-driven gene expression to counteract oxidative stress. Additionally, OSGIN1 has been associated with autophagy regulation and cellular senescence, suggesting roles in age-related pathologies. Studies link its dysregulation to neurodegenerative diseases, cancer progression, and cardiovascular disorders, highlighting its dual context-dependent roles as either a tumor suppressor or promoter.
Recombinant OSGIN1 protein is typically produced using bacterial (e.g., E. coli) or mammalian expression systems, followed by affinity chromatography purification. Its applications span in vitro studies exploring apoptosis mechanisms, drug screening for oxidative stress-related diseases, and functional validation in cellular models. As a research tool, it aids in deciphering mitochondrial dynamics, stress signaling crosstalk, and therapeutic targeting of pathways involving p53. Nrf2. or Bcl-2 proteins. Ongoing research focuses on its potential as a biomarker or therapeutic target in precision medicine approaches for cancer and degenerative conditions.
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