首页 / 产品 / 蛋白 / 信号通路蛋白

Recombinant Human SIK2 protein

  • 中文名: 盐诱导激酶2(SIK2)重组蛋白
  • 别    名: SIK2;KIAA0781;QIK;SNF1LK2;Serine/threonine-protein kinase SIK2
货号: PA1000-8880
Price: ¥询价
数量:
大包装询价

产品详情

纯度>90%SDS-PAGE.
种属Human
靶点SIK2
Uniprot No Q9H0K1
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-926aa
氨基酸序列MVMADGPRHLQRGPVRVGFYDIEGTLGKGNFAVVKLGRHRITKTEVAIKIIDKSQLDAVNLEKIYREVQIMKMLDHPHIIKLYQVMETKSMLYLVTEYAKNGEIFDYLANHGRLNESEARRKFWQILSAVDYCHGRKIVHRDLKAENLLLDNNMNIKIADFGFGNFFKSGELLATWCGSPPYAAPEVFEGQQYEGPQLDIWSMGVVLYVLVCGALPFDGPTLPILRQRVLEGRFRIPYFMSEDCEHLIRRMLVLDPSKRLTIAQIKEHKWMLIEVPVQRPVLYPQEQENEPSIGEFNEQVLRLMHSLGIDQQKTIESLQNKSYNHFAAIYFLLVERLKSHRSSFPVEQRLDGRQRRPSTIAEQTVAKAQTVGLPVTMHSPNMRLLRSALLPQASNVEAFSFPASGCQAEAAFMEEECVDTPKVNGCLLDPVPPVLVRKGCQSLPSNMMETSIDEGLETEGEAEEDPAHAFEAFQSTRSGQRRHTLSEVTNQLVVMPGAGKIFSMNDSPSLDSVDSEYDMGSVQRDLNFLEDNPSLKDIMLANQPSPRMTSPFISLRPTNPAMQALSSQKREVHNRSPVSFREGRRASDTSLTQGIVAFRQHLQNLARTKGILELNKVQLLYEQIGPEADPNLAPAAPQLQDLASSCPQEEVSQQQESVSTLPASVHPQLSPRQSLETQYLQHRLQKPSLLSKAQNTCQLYCKEPPRSLEQQLQEHRLQQKRLFLQKQSQLQAYFNQMQIAESSYPQPSQQLPLPRQETPPPSQQAPPFSLTQPLSPVLEPSSEQMQYSPFLSQYQEMQLQPLPSTSGPRAAPPLPTQLQQQQPPPPPPPPPPRQPGAAPAPLQFSYQTCELPSAASPAPDYPTPCQYPVDGAQQSDLTGPDCPRSPGLQEAPSSYDPLALSELPGLFDCEMLDAVDPQHNGYVLVN
预测分子量103,9 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于SIK2重组蛋白的3篇参考文献示例(注:文献信息为模拟概括,仅供参考):

1. **文献名称**:*"Recombinant SIK2 expression and its role in hepatic gluconeogenesis regulation"*

**作者**:Li, X., et al.

**摘要**:研究通过大肠杆菌系统重组表达SIK2蛋白,证实其通过磷酸化CRTC2调控肝脏糖异生,为代谢性疾病治疗提供靶点。

2. **文献名称**:*"Structural and functional characterization of salt-inducible kinase 2 (SIK2) using a mammalian cell expression system"*

**作者**:Park, J., & Kim, S.

**摘要**:利用哺乳动物细胞系统重组表达并纯化SIK2.解析其激酶结构域活性,揭示其与AMPK通路的相互作用机制。

3. **文献名称**:*"SIK2 recombinant protein inhibits ovarian cancer cell proliferation via modulating mTORC1 signaling"*

**作者**:Wang, Y., et al.

**摘要**:通过体外实验证明重组SIK2蛋白可抑制卵巢癌细胞mTORC1信号通路,为癌症治疗提供潜在策略。

4. **文献名称**:*"Development of a high-throughput screening assay for SIK2 inhibitors using recombinant protein"*

**作者**:Thompson, R., et al.

**摘要**:构建基于重组SIK2蛋白的高通量筛选平台,鉴定新型小分子抑制剂,用于代谢综合征药物开发。

(注:以上文献为示例,实际引用需查询PubMed、Web of Science等数据库获取真实信息。)

背景信息

SIK2 (salt-inducible kinase 2) is a serine/threonine protein kinase belonging to the AMP-activated protein kinase (AMPK) family. It plays critical roles in cellular energy homeostasis, metabolic regulation, and signal transduction pathways. Initially identified for its involvement in salt-induced hypertension research, SIK2 has since been linked to diverse physiological processes, including gluconeogenesis, lipid metabolism, and circadian rhythm regulation. Structurally, it contains a catalytic kinase domain and regulatory regions that mediate interactions with upstream activators like LKB1 or downstream substrates.

Recombinant SIK2 protein is typically produced using expression systems (e.g., E. coli, insect, or mammalian cells) for functional studies. Its production enables detailed investigation of enzymatic activity, substrate specificity, and inhibitor screening. Researchers often utilize purified SIK2 to explore its role in insulin signaling pathways, where it phosphorylates targets such as CRTC2/3 to modulate hepatic glucose production. Notably, SIK2 dysfunction has been implicated in metabolic disorders (e.g., obesity, type 2 diabetes), cancer progression through mTOR pathway interactions, and neurodegenerative conditions.

Recent interest in SIK2 stems from its therapeutic potential. Small-molecule inhibitors targeting SIK2 are being developed for metabolic syndrome and inflammation management. The recombinant protein also facilitates structural studies (e.g., X-ray crystallography) to map ATP-binding pockets and optimize drug design. However, challenges remain in understanding tissue-specific regulation and isoform diversity (SIK1/2/3). Current research increasingly employs CRISPR-edited cell models alongside recombinant SIK2 to dissect its complex regulatory networks and disease correlations.

客户数据及评论

折叠内容

大包装询价

×