| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OSBPL8 |
| Uniprot No | Q9BZF1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-889aa |
| 氨基酸序列 | MEGGLADGEPDRTSLLGDSKDVLGPSTVVANSDESQLLTPGKMSQRQGKEAYPTPTKDLHQPSLSPASPHSQGFERGKEDISQNKDESSLSMSKSKSESKLYNGSEKDSSTSSKLTKKESLKVQKKNYREEKKRATKELLSTITDPSVIVMADWLKIRGTLKSWTKLWCVLKPGVLLIYKTQKNGQWVGTVLLNACEIIERPSKKDGFCFKLFHPLEQSIWAVKGPKGEAVGSITQPLPSSYLIIRATSESDGRCWMDALELALKCSSLLKRTMIREGKEHDLSVSSDSTHVTFYGLLRANNLHSGDNFQLNDSEIERQHFKDQDMYSDKSDKENDQEHDESDNEVMGKSEESDTDTSERQDDSYIEPEPVEPLKETTYTEQSHEELGEAGEASQTETVSEENKSLIWTLLKQVRPGMDLSKVVLPTFILEPRSFLDKLSDYYYHADFLSEAALEENPYFRLKKVVKWYLSGFYKKPKGLKKPYNPILGETFRCLWIHPRTNSKTFYIAEQVSHHPPISAFYVSNRKDGFCLSGSILAKSKFYGNSLSAILEGEARLTFLNRGEDYVMTMPYAHCKGILYGTMTLELGGTVNITCQKTGYSAILEFKLKPFLGSSDCVNQISGKLKLGKEVLATLEGHWDSEVFITDKKTDNSEVFWNPTPDIKQWRLIRHTVKFEEQGDFESEKLWQRVTRAINAKDQTEATQEKYVLEEAQRQAARDRKTKNEEWSCKLFELDPLTGEWHYKFADTRPWDPLNDMIQFEKDGVIQTKVKHRTPMVSVPKMKHKPTRQQKKVAKGYSSPEPDIQDSSGSEAQSVKPSTRRKKGIELGDIQSSIESIKQTQEEIKRNIMALRNHLVSSTPATDYFLQQKDYFIIFLLILLQVIINFMFK |
| 预测分子量 | 101,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OSBPL8重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*"Expression and functional characterization of recombinant OSBPL8 in cholesterol transport"*
**作者**：Smith, J. et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组OSBPL8蛋白，证实其可特异性结合氧化固醇类分子。通过体外实验发现OSBPL8促进胆固醇从溶酶体向内质网的转运，并依赖其PH结构域与细胞膜相互作用。
2. **文献名称**：*"Structural insights into OSBPL8 lipid-binding domains using recombinant protein crystallography"*
**作者**：Zhang, L. et al.
**摘要**：利用昆虫细胞系统表达重组人源OSBPL8蛋白，解析其N端锚蛋白重复结构域的高分辨率晶体结构。研究表明该结构域介导与磷脂酰肌醇磷酸(PIPs)的结合，为OSBPL8参与膜动力学调控提供结构基础。
3. **文献名称**：*"OSBPL8 knockout cell model and rescue with recombinant protein reveals its role in autophagy regulation"*
**作者**：Watanabe, K. et al.
**摘要**：在OSBPL8基因敲除的HeLa细胞中，外源添加纯化的重组OSBPL8蛋白可恢复自噬小体形成缺陷，证明OSBPL8通过调控脂质筏微域稳定性影响自噬启动阶段。
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**备注**：上述文献为模拟示例，实际研究中建议通过PubMed或Web of Science以关键词“OSBPL8 recombinant”或“OSBPL8 protein purification”检索最新成果。OSBPL8作为脂代谢相关蛋白，其重组表达体系(原核/真核)及功能研究多集中于其脂质转运、膜接触位点形成等方向。
**Background of OSBPL8 Recombinant Protein**
OSBPL8 (Oxysterol-Binding Protein-Like 8), also known as ORP8. belongs to the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors involved in cholesterol homeostasis, membrane trafficking, and cell signaling. This protein is characterized by its N-terminal pleckstrin homology (PH) domain and a C-terminal OSBP-related ligand-binding domain (ORD), enabling interactions with phosphoinositides and oxysterols, respectively. OSBPL8 is predominantly expressed in tissues like the liver, brain, and macrophages, where it regulates lipid transport, membrane dynamics, and cellular responses to cholesterol fluctuations.
Recombinant OSBPL8 protein is engineered through molecular cloning and expression systems (e.g., *E. coli*, mammalian cells) to produce a purified, functional form for research. Its production enables detailed studies on lipid-protein interactions, subcellular localization, and mechanistic roles in diseases linked to lipid dysregulation, such as atherosclerosis, neurodegenerative disorders, and cancer. For instance, OSBPL8 has been implicated in modulating low-density lipoprotein (LDL) uptake and influencing endoplasmic reticulum (ER)-mitochondria communication, highlighting its potential as a therapeutic target.
Research using recombinant OSBPL8 has advanced structural analyses (e.g., crystallography) and functional assays (e.g., lipid-binding studies), shedding light on how its domains coordinate lipid sensing and trafficking. Additionally, it serves as a tool for screening small-molecule inhibitors or activators to manipulate cholesterol metabolism pathways. Overall, OSBPL8 recombinant protein is a critical resource for dissecting the molecular basis of lipid-related physiological and pathological processes.
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