| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOS1AP |
| Uniprot No | O75052 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-506aa |
| 氨基酸序列 | MPSKTKYNLVDDGHDLRIPLHNEDAFQHGICFEAKYVGSLDVPRPNSRVE IVAAMRRIRYEFKAKNIKKKKVSIMVSVDGVKVILKKKKKLLLLQKKEWT WDESKMLVMQDPIYRIFYVSHDSQDLKIFSYIARDGASNIFRCNVFKSKK KSQAMRIVRTVGQAFEVCHKLSLQHTQQNADGQEDGESERNSNSSGDPGR QLTGAERASTATAEETDIDAVEVPLPGNDVLEFSRGVTDLDAVGKEGGSH TGSKVSHPQEPMLTASPRMLLPSSSSKPPGLGTETPLSTHHQMQLLQQLL QQQQQQTQVAVAQVHLLKDQLAAEAAARLEAQARVHQLLLQNKDMLQHIS LLVKQVQELELKLSGQNAMGSQDSLLEITFRSGALPVLCDPTTPKPEDLH SPPLGAGLADFAHPAGSPLGRRDCLVKLECFRFLPPEDTPPPAQGEALLG GLELIKFRESGIASEYESNTDESEERDSWSQEELPRLLNVLQRQELGDGL DDEIAV |
| 预测分子量 | 83 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NOS1AP重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *"CAPON modulates cardiac repolarization via neuronal nitric oxide synthase signaling in the heart"*
**作者**: Wang Q, et al.
**摘要**: 本研究利用重组NOS1AP蛋白，揭示了其通过与神经元一氧化氮合酶(nNOS)相互作用调控心脏钾离子通道(如KCNH2)的机制，发现NOS1AP过表达可延长心肌细胞动作电位时程，提示其在心律失常中的潜在作用。
2. **文献名称**: *"Structural and functional characterization of the N-terminal domain of NOS1AP"*
**作者**: Li H, et al.
**摘要**: 通过表达并纯化NOS1AP的N端重组蛋白，结合X射线晶体学分析，揭示了其与突触后密度蛋白PSD-95的结合模式，阐明了NOS1AP在神经元信号转导中的结构基础。
3. **文献名称**: *"NOS1AP variants regulate protein expression and calcium handling in induced pluripotent stem cell-derived cardiomyocytes"*
**作者**: Chang KC, et al.
**摘要**: 研究利用重组NOS1AP蛋白及基因编辑技术，证明特定遗传变异通过影响NOS1AP与钙调蛋白的相互作用，导致心肌细胞钙稳态失调，为心脏疾病机制提供了新见解。
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以上文献涵盖了NOS1AP重组蛋白在心脏电生理、结构解析及疾病机制中的研究。如需更多文献，建议通过PubMed或Google Scholar以“NOS1AP recombinant protein”为关键词检索近年发表的文章。
NOS1AP (Nitric Oxide Synthase 1 Adaptor Protein), also known as CAPON, is a cytosolic protein that interacts with neuronal nitric oxide synthase (nNOS) to regulate nitric oxide (NO) signaling. This protein plays a critical role in cardiovascular and neurological systems by modulating ion channel activity, synaptic plasticity, and cellular stress responses. Its association with nNOS facilitates the spatial and functional coupling of NO production, influencing processes such as cardiac repolarization, neuronal development, and neurotransmission.
Recombinant NOS1AP proteins are engineered versions produced via molecular cloning and expression systems (e.g., E. coli, mammalian cells) for research and therapeutic applications. These proteins retain specific functional domains, such as the N-terminal phosphotyrosine-binding (PTB) domain and C-terminal PDZ-binding motif, enabling studies on protein-protein interactions and signaling mechanisms. Purification techniques like affinity chromatography ensure high purity and bioactivity.
Research on recombinant NOS1AP has advanced understanding of its pathological roles. Genetic variants in NOS1AP are linked to prolonged QT intervals (cardiac arrhythmia risk) and neuropsychiatric disorders like schizophrenia. Recombinant forms are used to investigate its interaction partners (e.g., Dexras1. synapsin) and downstream pathways, including MAPK/ERK and calcium signaling. Additionally, they serve as tools for drug screening, aiming to develop therapies targeting NOS1AP-related pathways in heart diseases or cognitive impairments.
Despite progress, questions remain about isoform-specific functions, post-translational modifications, and tissue-specific regulatory mechanisms. Recombinant NOS1AP continues to be vital for dissecting its dual roles in health and disease, bridging structural biology with translational medicine.
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