| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPHK1 |
| Uniprot No | Q9NYA1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-384aa |
| 氨基酸序列 | MDPAGGPRGV LPRPCRVLVL LNPRGGKGKA LQLFRSHVQP LLAEAEISFT LMLTERRNHA RELVRSEELG RWDALVVMSG DGLMHEVVNG LMERPDWETA IQKPLCSLPA GSGNALAASL NHYAGYEQVT NEDLLTNCTL LLCRRLLSPM NLLSLHTASG LRLFSVLSLA WGFIADVDLE SEKYRRLGEM RFTLGTFLRL AALRTYRGRL AYLPVGRVGS KTPASPVVVQ QGPVDAHLVP LEEPVPSHWT VVPDEDFVLV LALLHSHLGS EMFAAPMGRC AAGVMHLFYV RAGVSRAMLL RLFLAMEKGR HMEYECPYLV YVPVVAFRLE PKDGKGVFAV DGELMVSEAV QGQVHPNYFW MVSGCVEPPP SWKPQQMPPP EEPL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPHK1(鞘氨醇激酶1)重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Sphingosine kinase 1 regulates lysophosphatidic acid-mediated inflammatory responses in endothelial cells*
**作者**：Xia P. et al.
**摘要**：研究通过重组SPHK1蛋白实验，揭示了其在内皮细胞中介导炎症反应的作用机制，发现SPHK1通过调控鞘氨醇-1-磷酸(S1P)生成，促进炎症因子表达，参与动脉粥样硬化等病理过程。
2. **文献名称**：*Recombinant human sphingosine kinase 1 induces ovarian cancer cell proliferation through activation of the PI3K/AKT signaling pathway*
**作者**：Li W. et al.
**摘要**：利用重组人源SPHK1蛋白处理卵巢癌细胞，证实其通过激活PI3K/AKT通路促进细胞增殖和存活，提示SPHK1可能作为卵巢癌治疗的潜在靶点。
3. **文献名称**：*Targeting sphingosine kinase 1 (SphK1) in acute myeloid leukemia: role of recombinant SphK1 in leukemia cell survival and drug resistance*
**作者**：Sobue S. et al.
**摘要**：通过重组SPHK1蛋白实验，发现其过表达增强急性髓系白血病细胞的存活和化疗耐药性，抑制SPHK1活性可逆转耐药表型，为靶向治疗提供依据。
(注：以上文献信息为示例，实际引用需核对具体论文数据。)
**Background of Sphingosine Kinase 1 (SPHK1) Recombinant Protein**
Sphingosine kinase 1 (SPHK1) is a key enzyme in the sphingolipid signaling pathway, catalyzing the phosphorylation of sphingosine to produce sphingosine-1-phosphate (S1P), a bioactive lipid mediator. S1P regulates diverse cellular processes, including proliferation, survival, migration, and immune responses, by binding to specific G protein-coupled receptors (S1PRs) or acting intracellularly. SPHK1’s activity is tightly linked to pathological conditions such as cancer, inflammation, and metabolic disorders, making it a critical therapeutic target.
Recombinant SPHK1 protein is engineered using expression systems like *E. coli* or mammalian cells to ensure high purity and functional activity. This engineered protein retains the enzymatic properties of native SPHK1. enabling researchers to study its structure, kinetics, and interactions with inhibitors or activators *in vitro*. It serves as a vital tool for elucidating SPHK1’s role in disease mechanisms, screening potential drugs, and validating enzyme activity in experimental models.
In cancer research, SPHK1 overexpression is associated with tumor growth, angiogenesis, and chemotherapy resistance. Recombinant SPHK1 facilitates studies exploring its oncogenic signaling pathways, including crosstalk with growth factor receptors and downstream effectors like NF-κB or STAT3. Additionally, it aids in developing SPHK1 inhibitors, which show promise in preclinical studies for suppressing S1P-driven pathologies.
Overall, SPHK1 recombinant protein bridges fundamental research and translational applications, offering insights into sphingolipid biology and paving the way for novel therapeutic strategies.
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