| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CKMT1A |
| Uniprot No | P12532 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 40-417aa |
| 氨基酸序列 | ASERRRLYPPSAEYPDLRKHNNCMASHLTPAVYARLCDKTTPTGWTLDQCIQTGVDNPGHPFIKTVGMVAGDEETYEVFADLFDPVIQERHNGYDPRTMKHTTDLDASKIRSGYFDERYVLSSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQQQLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEKGGNMKRVFERFCRGLKEVERLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIKLPLLSKDSRFPKILENLRLQKRGTGGVDTAATGGVFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLERGQDIRIPTPVIHTKH |
| 预测分子量 | 47.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CKMT1A重组蛋白的3篇示例参考文献(注：部分内容为模拟示例，实际文献需通过学术数据库查询)：
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1. **文献名称**: *"Recombinant expression and functional characterization of human mitochondrial creatine kinase 1A in Escherichia coli"*
**作者**: Smith J, et al. (2015)
**摘要**: 该研究报道了在大肠杆菌中成功表达并纯化CKMT1A重组蛋白，优化了表达条件，分析了其酶动力学特性，并证明重组蛋白具有与天然蛋白相似的磷酸肌酸合成活性。
2. **文献名称**: *"Structural insights into CKMT1A oligomerization and its role in mitochondrial energy metabolism"*
**作者**: Zhang Y, et al. (2018)
**摘要**: 通过哺乳动物细胞系统表达重组CKMT1A，结合X射线晶体学解析其三维结构，揭示了其寡聚化机制，并探讨其在线粒体嵴形态维持和能量代谢中的功能。
3. **文献名称**: *"CKMT1A as a potential biomarker in colorectal cancer: Recombinant protein-based interaction studies"*
**作者**: Lee H, et al. (2020)
**摘要**: 利用重组CKMT1A蛋白进行蛋白质相互作用筛选，发现其与结直肠癌中高表达的ANT2蛋白存在直接结合，可能通过调节线粒体膜通透性影响肿瘤细胞存活。
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如需查找真实文献，建议在 **PubMed** 或 **Web of Science** 中检索关键词：
`CKMT1A recombinant`、`CKMT1A expression`、`mitochondrial creatine kinase 1A`。
CKMT1A (Creatine Kinase Mitochondrial 1A) is a key isoform of the creatine kinase (CK) enzyme family, primarily localized in the mitochondrial intermembrane space. It plays a critical role in cellular energy homeostasis by catalyzing the reversible transfer of a phosphoryl group between creatine and ATP, generating phosphocreatine—a high-energy reservoir that buffers ATP levels in tissues with fluctuating energy demands, such as cardiac muscle, skeletal muscle, and brain. This enzyme is encoded by the *CKMT1* gene, which undergoes alternative splicing to produce two major isoforms: CKMT1A (ubiquitous) and CKMT1B (testis-specific).
Structurally, CKMT1A exists as a homodimer, with each subunit containing a mitochondrial targeting sequence that directs its localization. Its activity is tightly linked to mitochondrial oxidative phosphorylation, facilitating efficient energy transfer between mitochondria and cytosolic ATP-consuming processes. Dysregulation of CKMT1A expression or function has been implicated in metabolic disorders, neurodegenerative diseases, and cardiac pathologies, highlighting its physiological and clinical relevance.
Recombinant CKMT1A protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Purified recombinant CKMT1A retains enzymatic activity, making it valuable for in vitro assays, drug screening, and mechanistic investigations of mitochondrial bioenergetics. Researchers also utilize it to explore CKMT1A’s role in disease models, develop diagnostic biomarkers, or engineer therapies targeting energy metabolism pathways. Its recombinant form ensures consistency for high-throughput studies while circumventing challenges associated with isolating the native protein from tissues.
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