| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PFK2 |
| Uniprot No | PFKFB3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-520aa |
| 氨基酸序列 | MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMKVRK QCALAALRDV KSYLAKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR ISCYEASYQP LDPDKCDRDL SLIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY LCRHGENEHN LQGRIGGDSG LSSRGKKFAS ALSKFVEEQN LKDLRVWTSQ LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVC THRERSEDAK KGPNPLMRRN SVTPLASPEP TKKPRINSFE EHVASTSAAL PSCLPPEVPT QLPGQNMKGS RSSADSSRKH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PFK2(磷酸果糖激酶-2)重组蛋白的参考文献示例及摘要概括：
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1. **文献名称**: *"Cloning and expression of rat liver 6-phosphofructo-2-kinase/fructose-2.6-bisphosphatase"*
**作者**: Sakakibara, R., et al.
**摘要**: 该研究报道了大鼠肝脏PFK2/FBPase2双功能酶的克隆及其在大肠杆菌中的重组表达，分析了该酶在调控果糖-2.6-二磷酸水平中的作用。
2. **文献名称**: *"Structural and functional studies of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2.6-bisphosphatase"*
**作者**: Okar, D.A., et al.
**摘要**: 通过重组人PFK2蛋白的晶体结构解析，阐明了其催化结构域的双功能机制，并揭示了磷酸化修饰对酶活性的调控。
3. **文献名称**: *"Regulation of 6-phosphofructo-2-kinase activity in isolated hepatocytes"*
**作者**: Rider, M.H., & Hue, L.
**摘要**: 利用重组表达的PFK2蛋白，验证了其在肝细胞糖代谢中的关键作用，并发现胰岛素和胰高血糖素通过磷酸化调控其活性。
4. **文献名称**: *"PFKFB3 in cancer: A dual role in tumorigenesis and therapeutic target"*
**作者**: Yalcin, A., et al.
**摘要**: 研究探讨了PFK2同工酶PFKFB3的重组蛋白在肿瘤细胞中的功能，发现其通过调控糖酵解促进癌细胞增殖，并提出靶向治疗的潜力。
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**注**：以上文献为示例，实际引用时需核实作者、年份及期刊信息。建议通过PubMed或Web of Science以关键词“PFK2 recombinant protein”或“PFKFB expression”检索最新研究。
**Background of PFK2 Recombinant Protein**
Phosphofructokinase-2 (PFK2), also known as 6-phosphofructo-2-kinase, is a bifunctional enzyme critical for regulating glycolysis and gluconeogenesis. It catalyzes the synthesis and degradation of fructose-2.6-bisphosphate (Fru-2.6-BP), a potent allosteric activator of phosphofructokinase-1 (PFK1) and inhibitor of fructose-1.6-bisphosphatase (FBPase). By modulating Fru-2.6-BP levels, PFK2 fine-tunes the balance between glycolytic flux and glucose production, adapting cellular metabolism to energy demands.
The PFK2 family includes four isoforms (PFKFB1–PFKFB4) with tissue-specific expression. For example, PFKFB3 is highly expressed in cancer cells, supporting the Warburg effect (aerobic glycolysis), while PFKFB2 is prominent in insulin-sensitive tissues like liver and muscle. Dysregulation of PFK2 activity is linked to metabolic disorders, including diabetes, obesity, and cancer, making it a therapeutic target.
Recombinant PFK2 proteins are engineered using expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to study their structure, kinetics, and regulatory mechanisms. These proteins enable researchers to investigate isoform-specific roles, post-translational modifications (e.g., phosphorylation by AMPK or insulin signaling pathways), and interactions with pharmacological inhibitors.
In drug discovery, recombinant PFK2 (especially PFKFB3) is used to screen inhibitors aiming to suppress cancer cell proliferation or correct metabolic imbalances. Its role in hypoxia adaptation and inflammation further expands its biomedical relevance. Overall, PFK2 recombinant proteins serve as essential tools for unraveling metabolic regulation and developing targeted therapies.
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