| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYB561D2 |
| Uniprot No | O14569 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-222aa |
| 氨基酸序列 | MALSAETESHIYRALRTASGAAAHLVALGFTIFVAVLARPGSSLFSWHPVLMSLAFSFLMTEALLVFSPESSLLHSLSRKGRARCHWVLQLLAPLCALLGLGLVILHKEQLGKAHLVTRHGQAGLLAVLWAGLQCSGGVGLLYPKLLPRWPLAKLKLYHATSGLVGYLLGSASLLLGMCSLWFTASVTGAAWYLAVLCPVLTSLVIMNQVSNAYLYRKRIQP |
| 分子量 | 50.16 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CYB561D2蛋白的参考文献示例(部分为假设性描述，供参考)：
1. **文献名称**: *Cloning and Functional Analysis of Recombinant Human CYB561D2 in Iron Metabolism*
**作者**: Smith, J. et al.
**摘要**: 本研究成功在大肠杆菌中表达重组人CYB561D2蛋白，证实其具有跨膜电子传递活性，可能通过调节细胞内的铁离子稳态参与氧化应激反应。
2. **文献名称**: *Structural Characterization of CYB561D2 Using X-ray Crystallography*
**作者**: Li, X. & Wang, Y.
**摘要**: 通过X射线晶体学解析了重组CYB561D2蛋白的三维结构，揭示了其血红素结合域的关键氨基酸残基，为理解其电子传递机制提供结构基础。
3. **文献名称**: *CYB561D2 Knockdown Alters Dopamine Beta-Hydroxylase Activity in Neuroendocrine Cells*
**作者**: Gomez, R. et al.
**摘要**: 利用重组CYB561D2蛋白研究其与多巴胺β-羟化酶的相互作用，发现CYB561D2可能通过调控还原当量供应影响儿茶酚胺合成。
4. **文献名称**: *Role of CYB561D2 in Tumor Cell Proliferation: Insights from Recombinant Protein Models*
**作者**: Chen, L. et al.
**摘要**: 在肿瘤细胞系中过表达重组CYB561D2后，观察到细胞增殖速率下降，提示该蛋白可能通过抑制ROS积累发挥抑癌作用。
注：以上文献内容为模拟示例，实际研究需以具体发表文章为准。建议通过PubMed或Web of Science以“CYB561D2”、“recombinant protein”为关键词检索最新文献。
CYB561D2 is a human protein belonging to the cytochrome b561 (CYB561) family, characterized by conserved transmembrane domains and heme-binding motifs critical for electron transfer. This protein is implicated in redox reactions, potentially mediating ascorbate-dependent transmembrane electron transport, a process linked to antioxidant defense and neurotransmitter metabolism. While its exact physiological role remains under investigation, CYB561D2 is hypothesized to regulate cellular iron homeostasis and reactive oxygen species (ROS) scavenging, particularly in secretory or neuroendocrine tissues where its expression is enriched. Dysregulation of CYB561D2 has been associated with neurological disorders and cancer, suggesting a role in disease pathogenesis.
Recombinant human CYB561D2 protein is engineered via heterologous expression systems (e.g., Escherichia coli or mammalian cells) to enable functional studies. Its recombinant form allows purification in soluble, active conformations, facilitating structural characterization, enzymatic assays, and interaction partner identification. Research on recombinant CYB561D2 has advanced understanding of its substrate specificity, pH-dependent activity, and involvement in cross-membrane electron shuttling. Recent studies explore its potential as a biomarker or therapeutic target, particularly in diseases involving oxidative stress or iron misregulation. However, mechanistic details, including its precise subcellular localization and regulatory pathways, require further elucidation. Recombinant protein tools remain pivotal in unraveling CYB561D2's biological and pathological significance.
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