| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYB5D1 |
| Uniprot No | Q6P9G0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-228aa |
| 氨基酸序列 | MPRRGLVAGP DLEYFQRRYF TPAEVAQHNR PEDLWVSYLG RVYDLTSLAQ EYKGNLLLKP IVEVAGQDIS HWFDPKTRDI RKHIDPLTGC LRYCTPRGRF VHVPPQLPCS DWANDFGKPW WQGSYYEVGR LSAKTRSIRI INTLTSQEHT LEVGVLESIW EILHRYLPYN SHAASYTWKY EGKNLNMDFT LEENGIRDEE EEFDYLSMDG TLHTPAILLY FNDDLTEL |
| 分子量 | 26.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CYB5D1蛋白的示例参考文献(内容为示例性质，建议通过学术数据库核实真实文献)：
1. **"Functional characterization of recombinant human CYB5D1 in mitochondrial electron transfer"**
*Author: Li X, et al.*
*摘要*：本研究通过大肠杆菌系统成功表达了重组人CYB5D1蛋白，并验证其在线粒体电子传递链中的潜在作用，表明CYB5D1可能通过调节氧化还原反应影响细胞凋亡。
2. **"Structural insights into human CYB5D1 protein and its interaction with cytochrome P450 enzymes"**
*Author: Smith J, et al.*
*摘要*：利用X射线晶体学解析了重组人CYB5D1的三维结构，揭示了其与细胞色素P450酶的相互作用界面，为理解其在脂质代谢和药物代谢中的调控机制提供依据。
3. **"CYB5D1 overexpression suppresses tumor growth via ROS-mediated apoptosis in hepatocellular carcinoma"**
*Author: Wang Y, et al.*
*摘要*：通过哺乳动物细胞表达系统制备重组CYB5D1蛋白，发现其过表达可诱导活性氧(ROS)积累，从而促进肝癌细胞凋亡，提示其潜在的抑癌功能。
4. **"Optimization of recombinant CYB5D1 expression in Pichia pastoris and its application in drug metabolism studies"**
*Author: Gonzalez R, et al.*
*摘要*：在毕赤酵母中优化CYB5D1的分泌表达，纯化后蛋白用于体外药物代谢实验，证明其对特定P450酶活性具有增强作用。
**注意**：以上文献信息为模拟示例，实际文献需通过PubMed、Web of Science或Google Scholar等平台检索确认。建议结合关键词"recombinant human CYB5D1"和"cytochrome b5 domain-containing protein 1"进行精准查询。
Recombinant human CYB5D1 (Cytochrome b5 domain-containing protein 1) is a less characterized member of the cytochrome b5 superfamily, which typically participates in redox reactions and electron transfer. The CYB5D1 gene encodes a 149-amino acid protein featuring a conserved cytochrome b5-like domain at its N-terminus, anchored by a hydrophobic transmembrane segment. Unlike canonical cytochrome b5 isoforms, CYB5D1 lacks a C-terminal endoplasmic reticulum (ER) retention signal and may localize to mitochondria or other subcellular compartments, though its precise cellular distribution remains under investigation. Functionally, CYB5D1 is hypothesized to interact with electron transport chains or redox-sensitive enzymes, potentially influencing lipid metabolism, drug detoxification, or iron homeostasis. Recent studies link its expression to cancer progression, with elevated levels observed in certain tumors, suggesting roles in cell proliferation or apoptosis. However, mechanistic insights are limited due to low endogenous expression and technical challenges in protein isolation. Recombinant CYB5D1 production, typically achieved via bacterial or mammalian expression systems, enables structural studies, antibody development, and functional assays to elucidate its interactions and biochemical properties. Its recombinant form is crucial for exploring its involvement in metabolic disorders, neurodegenerative diseases, or cancer biology, providing a foundation for diagnostic or therapeutic applications. Further research is needed to clarify its physiological partners and pathological significance.
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