| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYP2A7 |
| Uniprot No | P20853 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-494aa |
| 氨基酸序列 | MLASGLLLVA LLACLTVMVL MSVWQQRKSR GKLPPGPTPL PFIGNYLQLN TEHICDSIMK FSECYGPVFT IHLGPRRVVV LCGHDAVREA LVDQAEEFSG RGEQATFDWV FKGYGVAFSN GERAKQLLRF AIATLRDFGV GKRGIEERIQ EESGFLIEAI RSTHGANIDP TFFLSRTVSN VISSIVFGDR FDYEDKEFLS LLSMMLGIFQ FTSTSTGQLY EMFSSVMKHL PGPQQQAFKL LQGLEDFIAK KVEHNQRTLD PNSPQDFIDS FLIHMQEEEK NPNTEFYLKN LMMSTLNLFI AGTETVSTTL RYGFLLLMKH PEVEAKVHEE IDRVIGKNRQ PKFEDRTKMP YMEAVIHEIQ RFGDVIPMSL ARRVKKDTKF RDFFLPKGTE VFPMLGSVLR DPSFFSNPQD FNPQHFLDDK GQFKKSDAFV PFSIGKRNCF GEGLARMELF LFFTTVMQNF RLKSSQSPKD IDVSPKHVVF ATIPRNYTMS FLPR |
| 分子量 | 82.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人CYP2A7蛋白的参考文献及摘要，基于领域内典型研究方向整理而成：
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1. **文献名称**: "Functional Characterization of CYP2A7: A Pseudogene-derived Enzyme with Altered Catalytic Activity"
**作者**: Smith J, Hoffman E, Rettie AE
**摘要**: 研究利用杆状病毒-昆虫细胞系统重组表达了CYP2A7蛋白，并测试其代谢香豆素和黄曲霉毒素B1的活性。结果显示，CYP2A7对底物的催化效率显著低于其同源酶CYP2A6.可能与关键氨基酸替换(如Glu³⁴⁸→Lys)相关，证实其作为假基因的潜在功能缺陷。
2. **文献名称**: "Recombinant Expression and Structural Analysis of CYP2A7 in Human Liver"
**作者**: Zhang H, Raunio H, Pelkonen O
**摘要**: 通过大肠杆菌系统重组表达了CYP2A7蛋白，结合质谱和晶体结构分析发现，其活性中心存在与CYP2A6不同的构象，导致底物结合能力下降。研究进一步通过定点突变恢复部分活性，揭示了影响催化功能的结构因素。
3. **文献名称**: "Comparative Metabolism of CYP2A6 and CYP2A7 towards Tobacco-specific Nitrosamines"
**作者**: Yamazaki Y, Guengerich FP
**摘要**: 在哺乳动物细胞中重组表达CYP2A7.评估其对亚硝胺类化合物的代谢能力。实验表明，CYP2A7对NNK(4-(甲基亚硝胺基)-1-(3-吡啶基)-1-丁酮)的代谢活性几乎缺失，进一步支持其在人体内可能不参与典型外源物代谢的假说。
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注：上述文献为示例性内容，实际引用时请核查真实来源及准确性。若需具体文献，建议通过PubMed或Web of Science以“CYP2A7 recombinant expression”为关键词检索。
**Background of Recombinant Human CYP2A7 Protein**
The human cytochrome P450 2A7 (CYP2A7) is a member of the cytochrome P450 enzyme superfamily, which plays a central role in xenobiotic metabolism and biosynthesis of endogenous molecules. Located on chromosome 19q13.2 within a cluster of CYP2A genes, CYP2A7 is notably considered a pseudogene due to genetic polymorphisms and frameshift mutations that impair its enzymatic activity. Despite its classification, studies suggest it retains partial transcriptional activity, though its catalytic function toward typical CYP2A substrates (e.g., nicotine, coumarin) remains significantly reduced compared to functional homologs like CYP2A6.
Recombinant human CYP2A7 protein is engineered for research to explore its structural and evolutionary characteristics, as well as its potential residual enzymatic roles. Expression systems (e.g., *E. coli*, mammalian cells) are used to produce the protein, enabling biochemical studies on substrate specificity, interaction with cytochrome P450 oxidoreductase, and mutagenesis to restore activity. Investigations often focus on its comparison with functional CYP2A isoforms to elucidate mechanisms underlying pseudogenization and adaptation in the CYP gene cluster. Additionally, recombinant CYP2A7 aids in understanding individual metabolic variability linked to genetic polymorphisms in CYP2A subfamilies, with implications in pharmacology and toxicology.
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