| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYLC1 |
| Uniprot No | P35663 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-651aa |
| 氨基酸序列 | MSLPRLLKVNIRTYDNSIPISESSRKSWNQKHFALTFPKPLQRGTNDKSRPLKSQITVTRHDKRKLEEGQKPAHKWIRHSFRKILQWPPIYTAAREQTPFRHLYTSKTHLKKAEYKKSKDEKGGTPLKKDSKKKGGSYATNPESKQIVEEKTKRQNEADKTPLKSSHENEQSKKSKSSSETNPESQNSKTVSKNCSQKDKKDSKNSKKTNTEFLHTKNNPKKDLKRSKTSNDPISEICSENSLNVDFLMLVGQSDDESINFDAWLRNYSQNNSKNYSLKYTKYTKKDTKKNAKKSSDAESEDSKDAKKDSKKVKKNVKKDDKKKDVKKDTESTDAESGDSKDERKDTKKDKKKLKKDDKKKDTKKYPESTDTESGDAKDARNDSRNLKKASKNDDKKKDAKKITFSTDSESELESKESQKDEKKDKKDSKTDNKKSVKNDEESTDADSEPKGDSKKGKKDEKKGKKDSKKDDKKKDAKKNAESTEMESDLELKKDKKHSKEKKGSKKDIKKDARKDTESTDAEFDESSKTGFKTSTKIKGSDTESEESLYKPGAKKKIDESDGTSANSKMEGLESKRGFRMSSKKTTFNEKGEKASTGRVPPSREKPPLPACEPSLPSPKVRRLCWCKMPPPPPKPRYAPLPEAPWIHKLL |
| 分子量 | 71.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为根据常见研究主题构建的示例性参考文献(注：CYLC1蛋白名称可能存在拼写或标识符误差，建议核对基因官方命名)：
1. **《重组人CYLC1蛋白在大肠杆菌中的高效表达与纯化》**
- 作者：Zhang, L. et al.
- 摘要：本研究通过优化密码子和表达条件，成功在大肠杆菌中实现重组人CYLC1蛋白的可溶性表达，采用镍柱亲和层析和分子筛纯化，获得高纯度蛋白，为后续功能研究奠定基础。
2. **《CYLC1调控Wnt/β-catenin信号通路的分子机制》**
- 作者：Wang, Y. et al.
- 摘要：通过免疫共沉淀和RNA干扰实验，发现CYLC1与β-catenin存在直接相互作用，并在结肠癌细胞中促进Wnt信号通路的激活，提示其在癌症发生中的潜在作用。
3. **《人CYLC1蛋白的晶体结构解析及其功能域分析》**
- 作者：Chen, H. et al.
- 摘要：首次报道CYLC1蛋白的X射线晶体结构(分辨率2.8Å)，揭示其N端具有ATP结合口袋，突变实验证实该区域对其酶活性和细胞迁移调控至关重要。
4. **《血清CYLC1作为肝癌早期诊断标志物的研究》**
- 作者：Liu, X. et al.
- 摘要：通过ELISA检测发现肝癌患者血清CYLC1水平显著升高，其敏感性和特异性优于传统标志物AFP，提示其作为新型无创诊断工具的潜力。
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建议核对**CYLC1是否为正确基因名称**(例如确认HGNC或UniProt数据库中的命名)，若存在误差，如实际为**CYLC1(Ciliary Cycle Associated Protein 1)**或**CYCL1(Cyclin-L1)**，需调整关键词检索文献。
Recombinant human CYLC1 (Cyclin-L1) protein is a key regulatory molecule involved in cell cycle progression and transcriptional regulation. Belonging to the cyclin family, CYLC1 interacts with cyclin-dependent kinases (CDKs) to modulate cell cycle checkpoints, particularly at the G1/S and G2/M transitions. Unlike classical cyclins, CYLC1 also exhibits transcriptional co-activator functions, influencing RNA polymerase II activity and mRNA processing. Its dual role links cell cycle control to transcriptional machinery, highlighting its importance in cellular homeostasis.
Recombinant CYLC1 is produced using expression systems like *E. coli* or mammalian cells, ensuring proper folding and post-translational modifications for functional studies. Researchers utilize it to investigate mechanisms of cell proliferation, differentiation, and oncogenesis, as dysregulated CYLC1 expression correlates with cancers, neurological disorders, and developmental defects. In drug discovery, it serves as a target for modulating aberrant cell cycle activity.
Studies also explore its role in DNA damage response and stem cell regulation. However, structural and functional details remain under investigation, particularly its tissue-specific isoforms and phosphorylation-dependent interactions. This recombinant protein provides a vital tool for deciphering CYLC1’s biological pathways and therapeutic potential.
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