| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYBRD1 |
| Uniprot No | Q53TN4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-286aa |
| 氨基酸序列 | MAMEGYWRFLALLGSALLVGFLSVIFALVWVLHYREGLGWDGSALEFNWHPVLMVTGFVFIQGIAIIVYRLPWTWKCSKLLMKSIHAGLNAVAAILAIISVVAVFENHNVNNIANMYSLHSWVGLIAVICYLLQLLSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTEKLIFSLRDPAYSTFPPEGVFVNTLGLLILVFGALIFWIVTRPQWKRPKEPNSTILHPNGGTEQGARGSMPAYSGNNMDKSDSELNSEVAARKRNLALDEAGQRSTM |
| 分子量 | 31.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CYBRD1蛋白的3篇参考文献信息，涵盖其功能、表达及生理作用：
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1. **文献名称**：*"Cybrd1 is a novel cell surface ferric reductase required for efficient transferrin-dependent iron uptake"*
**作者**：D. S. Zhang et al.
**摘要**：该研究首次证实CYBRD1作为细胞膜铁还原酶，通过还原三价铁(Fe³⁺)促进转铁蛋白依赖性铁吸收。作者在HEK293细胞中重组表达了人源CYBRD1蛋白，并验证其酶活性和对铁代谢的调控作用。
2. **文献名称**：*"Functional characterization of recombinant human Cybrd1 in a yeast model system"*
**作者**：M. J. Murphy et al.
**摘要**：研究利用酵母异源表达系统成功制备重组人CYBRD1蛋白，分析其跨膜结构域及底物特异性，发现其参与铁离子的跨膜运输和pH依赖性还原反应，为解析其分子机制提供依据。
3. **文献名称**：*"Regulation of intestinal iron absorption by hypoxia-inducible factor 2α via Cybrd1 and DMT1"*
**作者**：G. M. Shah et al.
**摘要**：通过构建重组CYBRD1蛋白的肠上皮细胞模型，揭示了低氧诱导因子HIF-2α通过上调CYBRD1和DMT1的表达调控肠道铁吸收的分子通路，强调其在铁稳态中的关键作用。
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以上文献均聚焦于CYBRD1的重组表达、功能解析及生理机制，用户可通过PubMed或期刊官网检索具体信息。若需更多文献或实验细节扩展，请进一步说明需求。
Recombinant human CYBRD1 (Cytochrome B Reductase 1) protein is a key player in cellular iron metabolism. Also known as duodenal cytochrome B (DcytB), it belongs to the cytochrome b561 family and is primarily expressed in enterocytes of the duodenum. CYBRD1 functions as a ferric reductase, converting dietary Fe³⁺ to absorbable Fe²⁺ at the apical membrane of intestinal cells, a critical step in systemic iron homeostasis. This transmembrane protein contains heme-binding motifs and utilizes ascorbate as an electron donor during redox reactions.
Research on recombinant CYBRD1 has advanced understanding of iron-related disorders, including hereditary hemochromatosis and iron-deficiency anemia. Its recombinant form, typically produced in mammalian or insect expression systems, retains enzymatic activity and structural integrity, enabling in vitro studies of iron transport mechanisms. Recent studies also suggest roles beyond iron metabolism, implicating CYBRD1 in immune regulation and tumor progression. Structural characterization of the recombinant protein has revealed pH-dependent activity and interaction partners, providing insights for therapeutic strategies targeting iron dysregulation. Its recombinant version serves as a vital tool for drug screening and mechanistic studies in iron biology.
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