| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYB5R4 |
| Uniprot No | Q7L1T6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-521aa |
| 氨基酸序列 | MLNVPSQSFPAPRSQQRVASGGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGRLIEVTEEELKKHNKKDDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGSDGTELFDQVHRWVNYESMLKECLVGRMAIKPAVLKDYREEEKKVLNGMLPKSQVTDTLAKEGPSYPSYDWFQTDSLVTIAIYTKQKDINLDSIIVDHQNDSFRAETIIKDCLYLIHIGLSHEVQEDFSVRVVESVGKIEIVLQKKENTSWDFLGHPLKNHNSLIPRKDTGLYYRKCQLISKEDVTHDTRLFCLMLPPSTHLQVPIGQHVYLKLPITGTEIVKPYTPVSGSLLSEFKEPVLPNNKYIYFLIKIYPTGLFTPELDRLQIGDFVSVSSPEGNFKISKFQELEDLFLLAAGTGFTPMVKILNYALTDIPSLRKVKLMFFNKTEDDIIWRSQLEKLAFKDKRLDVEFVLSAPISEWNGKQGHISPALLSEFLKRNLDKSKVLVCICGPVPFTEQGVRLLHDLNFSKNEIHSFTA |
| 分子量 | 85.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CYB5R4蛋白的模拟参考文献示例(仅供格式参考，非真实文献)：
1. **标题**：《重组人CYB5R4蛋白的表达及其对脂质代谢的调控作用》
**作者**：Smith A, et al.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化了重组人CYB5R4蛋白，发现其能够通过NADH依赖的还原酶活性调节细胞内脂质微环境，提示其在胆固醇合成通路中的潜在功能。
2. **标题**：《人源CYB5R4的晶体结构揭示其催化机制》
**作者**：Zhang L, Wang Y.
**摘要**：首次报道了CYB5R4蛋白的X射线晶体结构，揭示了其底物结合口袋及电子传递路径，为开发针对该酶的靶向药物提供了结构基础。
3. **标题**：《CYB5R4在神经退行性疾病中的异常表达及功能研究》
**作者**：Chen H, et al.
**摘要**：发现阿尔茨海默病患者的脑组织中CYB5R4表达显著下调，重组蛋白体外实验表明其缺失可能通过氧化应激途径加剧神经元损伤。
4. **标题**：《昆虫细胞系统中重组CYB5R4的高效表达与酶动力学分析》
**作者**：Johnson R, et al.
**摘要**：利用杆状病毒-昆虫细胞系统实现CYB5R4的高效分泌表达，并系统分析了其最适pH、温度及辅酶特异性等酶学性质。
**注**：以上为示例文献，实际引用请通过**PubMed**或**Web of Science**查询最新研究，并核实文献真实性。
Recombinant human CYB5R4 protein is a genetically engineered form of the cytochrome b5 reductase 4 enzyme, which belongs to the NADH-cytochrome b5 reductase family. CYB5R4 is a membrane-associated flavoprotein involved in cellular redox processes, primarily catalyzing the transfer of electrons from NADH to cytochrome b5. This activity supports critical metabolic pathways, including fatty acid desaturation, cholesterol biosynthesis, and drug metabolism. Unlike its isoform CYB5R3. which exists in both soluble and membrane-bound forms, CYB5R4 is predominantly localized to the endoplasmic reticulum and Golgi apparatus, suggesting specialized roles in organelle-specific redox regulation. Its expression is linked to cellular stress responses and lipid homeostasis.
The recombinant protein is produced via heterologous expression systems (e.g., E. coli or mammalian cells) to enable functional and structural studies. Researchers utilize it to investigate mechanisms underlying diseases associated with redox imbalances, such as neurodegenerative disorders, cancer, and metabolic syndromes. Structural studies of recombinant CYB5R4 aim to elucidate its FAD-binding domain and membrane-interaction motifs, providing insights into enzymatic regulation and potential therapeutic targeting. Its applications also extend to in vitro assays for drug screening and enzyme kinetics analysis, highlighting its versatility in biomedical research.
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