| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYB5-M |
| Uniprot No | O43169 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-146aa |
| 氨基酸序列 | MATAEASGSDGKGQEVETSVTYYRLEEVAKRNSLKELWLVIHGRVYDVTRFLNEHPGGEEVLLEQAGVDASESFEDVGHSSDAREMLKQYYIGDIHPSDLKPESGSKDPSKNDTCKSCWAYWILPIIGAVLLGFLYRYYTSESKSS |
| 分子量 | 42.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CYB5-M蛋白的模拟参考文献示例(虚构内容，供参考)：
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1. **标题**：*"Expression and Purification of Recombinant Human CYB5-M in Escherichia coli: Optimization and Functional Analysis"*
**作者**：Smith, J.R., Lee, T., & Chen, W.
**摘要**：本研究报道了人源线粒体细胞色素b5(CYB5-M)在大肠杆菌中的重组表达优化策略，采用His标签纯化系统获得高纯度蛋白，并验证其与细胞色素P450酶(CYP450)的相互作用，证实其在药物代谢中的还原辅酶功能。
2. **标题**：*"Structural Insights into Human CYB5-M: X-ray Crystallography and Mutagenesis Studies"*
**作者**：Gomez, A., Patel, S., & Zhang, Y.
**摘要**：通过X射线晶体学解析了CYB5-M的2.1 Å分辨率结构，揭示了其血红素结合域的关键氨基酸残基，并利用定点突变实验证明第65位组氨酸对蛋白稳定性和电子传递能力的决定性作用。
3. **标题**：*"Role of Recombinant CYB5-M in Fatty Acid Desaturation: In Vitro Reconstitution System"*
**作者**：Kim, H., Ito, M., & Wang, L.
**摘要**：构建了包含重组CYB5-M、NADH-细胞色素b5还原酶和硬脂酰-CoA去饱和酶的体外系统，证明CYB5-M在催化油酸生成过程中不可或缺，为脂代谢疾病研究提供模型。
4. **标题**：*"Recombinant CYB5-M as a Therapeutic Target in Cancer: Interaction with Cytochrome c Oxidase"*
**作者**：Rodriguez, P., Nguyen, V., & Kumar, S.
**摘要**：发现CYB5-M在癌症细胞线粒体中高表达，其通过调控细胞色素c氧化酶活性影响ROS生成，重组蛋白的抑制作用可降低肿瘤细胞增殖，提示其作为抗癌靶点的潜力。
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*注：以上为虚构参考文献，实际引用需查询PubMed、Web of Science等数据库获取真实文献。*
Recombinant human CYB5-M (cytochrome b5 mitochondrial isoform) is a heme-binding protein involved in electron transfer reactions within mitochondria. Belonging to the cytochrome b5 family, CYB5-M is membrane-anchored and primarily located in the mitochondrial outer membrane. It plays a critical role in lipid metabolism, including fatty acid desaturation and elongation, by transferring electrons to enzymes such as stearyl-CoA desaturase. Additionally, CYB5-M interacts with cytochrome P450 enzymes to support oxidative reactions in drug metabolism and steroidogenesis.
The recombinant form is produced using engineered expression systems (e.g., *E. coli* or yeast), enabling high-purity, soluble protein for biochemical studies. Its N-terminal membrane-binding domain is often modified to enhance solubility. Research on CYB5-M focuses on its structural interactions with redox partners, roles in metabolic disorders (e.g., anemia, cancer drug resistance), and potential therapeutic applications. Genetic mutations in CYB5M are linked to type II methemoglobinemia, driving interest in its functional mechanisms. Recombinant CYB5-M serves as a vital tool for elucidating mitochondrial redox biology and developing treatments for metabolic diseases.
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