| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYBA |
| Uniprot No | P13498 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-195aa |
| 氨基酸序列 | GQIEWAMWA NEQALASGLI LITGGIVATA GRFTQWYFGA YSIVAGVFVC LLEYPRGKRK KGSTMERWGQ KYMTAVVKLF GPFTRNYYVR AVLHLLLSVP AGFLLATILG TACLAIASGI YLLAAVRGEQ WTPIEPKPRE RPQIGGTIKQ PPSNPPPRPP AEARKKPSEE EAAVAAGGPP GGPQVNPIPV TDEVV |
| 分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CYBA蛋白的3篇参考文献概览：
1. **文献名称**: *"Expression and characterization of recombinant human p22phox: Role of N-glycosylation and disulfide bonding in flavocytochrome b558"*
**作者**: Smith J.R., Li X., Parkos C.A.
**摘要**: 该研究在大肠杆菌和哺乳动物细胞系统中表达了重组人CYBA(p22phox)，发现N-糖基化和二硫键对其与gp91phox亚基结合形成功能性细胞色素b558复合物至关重要，并揭示了其结构对NADPH氧化酶活性的调控作用。
2. **文献名称**: *"Functional analysis of CYBA mutations in chronic granulomatous disease using a novel recombinant protein system"*
**作者**: Ueyama T., Nakakita Y., Nakamura T., et al.
**摘要**: 通过杆状病毒-昆虫细胞系统表达重组人CYBA蛋白，结合慢性肉芽肿病(CGD)患者的突变体分析，发现特定结构域突变导致p22phox/gp91phox相互作用缺陷，阐释了CYBA突变致病的分子机制。
3. **文献名称**: *"Probing the role of p22phox in NADPH oxidase assembly: Insights from in vitro reconstitution assays"*
**作者**: Clark R.A., Volkman B.F., Ortiz de Montellano P.R.
**摘要**: 使用纯化的重组人CYBA和gp91phox蛋白进行体外重组实验，证实CYBA作为支架蛋白对氧化酶复合体膜转运及稳定性起关键作用，并通过表面等离子体共振技术(SPR)定量分析了亚基间结合动力学。
---
以上文献均为示例性概括，实际文献需通过学术数据库(如PubMed/Web of Science)检索确认。建议使用关键词“recombinant human CYBA/p22phox”或结合研究主题(如“NADPH oxidase assembly”“mutagenesis”)进一步筛选。
Cytochrome b-245 alpha chain (CYBA), also known as p22phox, is a critical subunit of the NADPH oxidase complex, a key enzyme system responsible for reactive oxygen species (ROS) generation in phagocytic and non-phagocytic cells. Encoded by the CYBA gene in humans, this 22 kDa transmembrane protein partners with NOX isoforms (e.g., NOX1-5) to form functional oxidase complexes. It stabilizes NOX proteins and facilitates electron transfer during superoxide production, essential for microbial killing, inflammatory signaling, and redox-regulated cellular processes.
Mutations in CYBA are linked to autosomal recessive chronic granulomatous disease (CGD), a rare immunodeficiency disorder characterized by defective NADPH oxidase activity, leading to recurrent infections and granuloma formation. Recombinant human CYBA protein, typically produced via bacterial or mammalian expression systems, enables mechanistic studies of NADPH oxidase assembly/activation and its role in pathologies like cardiovascular diseases, neurodegeneration, and cancer. Its structural analysis (e.g., conserved heme-binding histidines) and interaction networks are vital for developing targeted therapies. Research on recombinant CYBA also aids in screening small-molecule modulators of ROS production and validating gene therapy approaches for CGD.
×
