| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYB5 |
| Uniprot No | P00167 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-134aa |
| 氨基酸序列 | MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPETLITTIDSSSSWWTNWVIPAISAVAVALMYRLYMAED |
| 分子量 | 15.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CYB5蛋白的3篇参考文献简介，涵盖结构研究、功能应用及疾病关联方向：
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1. **文献名称**：*"Structural and functional characterization of recombinant human cytochrome b5"*
**作者**：Sono M et al.
**摘要**：通过X射线晶体学和光谱学解析了重组人CYB5的三维结构，发现其血红素结合域对电子传递效率的关键作用，并验证了其与细胞色素P450酶的高效相互作用机制。
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2. **文献名称**：*"Expression and purification of functional human cytochrome b5 in Escherichia coli for drug metabolism studies"*
**作者**：Zhang Y et al.
**摘要**：开发了一种高效的大肠杆菌表达系统，获得可溶性的重组人CYB5蛋白，验证其在体外药物代谢模型中可显著增强细胞色素P450酶对多种药物的氧化代谢活性。
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3. **文献名称**：*"Role of cytochrome b5 in modulating ferroptosis: Implications in cancer therapy"*
**作者**：Chen L et al.
**摘要**：发现重组人CYB5通过调节脂质过氧化水平和铁代谢通路抑制铁死亡(ferroptosis)，揭示了CYB5在肿瘤细胞耐药性中的潜在作用，为靶向CYB5的抗癌策略提供依据。
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**备注**：上述文献的标题及摘要为简化模拟，实际研究中可能需要通过PubMed、Web of Science等平台检索具体论文(关键词：recombinant human cytochrome b5. function, structure, disease)。如需获取全文，建议访问学术数据库。
Recombinant human CYB5 (cytochrome b5) protein is a small heme-binding protein playing critical roles in various cellular redox processes. Naturally occurring in three isoforms—membrane-bound, soluble, and mitochondrial—CYB5 is ubiquitously expressed, with the membrane-bound form localized to the endoplasmic reticulum and plasma membrane, while the soluble form is found in erythrocytes. The mitochondrial isoform is embedded in the outer mitochondrial membrane.
CYB5 acts as an electron donor in enzymatic reactions, notably supporting cytochrome P450 (CYP) enzymes during drug metabolism, steroidogenesis, and fatty acid desaturation. It facilitates electron transfer from NADH via CYB5 reductase, influencing lipid metabolism, including elongation and desaturation of fatty acids, and heme degradation. Its interaction with CYP enzymes enhances catalytic efficiency in oxidations, such as cholesterol biosynthesis and detoxification pathways.
Recombinant CYB5. typically produced in *E. coli* or mammalian expression systems, enables detailed biochemical and structural studies. Its applications span drug discovery, metabolic disease research, and enzyme replacement strategies. Mutations or deficiencies in CYB5 are linked to rare disorders, including hereditary methemoglobinemia and lipid metabolism abnormalities, underscoring its physiological importance. Engineered variants also aid in investigating metabolic networks and developing targeted therapies.
Overall, recombinant CYB5 serves as a vital tool for dissecting redox biology and advancing therapeutic interventions for metabolic and genetic disorders.
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