| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DAZ3 |
| Uniprot No | Q9NR90 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-486aa |
| 氨基酸序列 | MSAANPETPN STISREASTQ SSSAAASQGW VLPEGKIVPN TVFVGGIDAR MDETEIGSCF GRYGSVKEVK IITNRTGVSK GYGFVSFVND VDVQKIVGSQ IHFHGKKLKL GPAIRKQKLC ARHVQPRPLV VNPPPPPQFQ NVWRNPNTET YLQPQITPNP VTQHVQAYSA YPHSPGQVIT GCQLLVYNYQ EYPTYPDSAF QVTTGYQLPV YNYQPFPAYP RSPFQVTAGY QLPVYNYQAF PAYPNSPFQV ATGYQFPVYN YQPFPAYPSS PFQVTAGYQL PVYNYQAFPA YPNSPFQVAT GYQFPVYNYQ AFPAYPNSPV QVTTGYQLPV YNYQAFPAYP NSPFQVATGY QFPVYNYQAF PAYPNSPVQV TTGYQLPVYN YQAFPAYPNS PFQVATGYQF PVYNYQAFPA YPNSPVQVTT GYQLPVYNYQ AFPAYPNSAV QVTTGYQFHV YNYQMPPQCP VGEQRRNLWT EAYKWWYLVC LIQRRD |
| 分子量 | 54.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DAZ3蛋白的虚构参考文献示例，基于相关领域常见研究方向整理，仅供格式参考：
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1. **文献名称**：Expression and Purification of Recombinant Human DAZ3 Protein in Escherichia coli
**作者**：Chen, L., Wang, X., & Liu, Y.
**摘要**：本研究报道了人DAZ3基因在大肠杆菌中的克隆与表达，利用His标签亲和层析纯化重组蛋白，并通过Western blot和质谱验证其正确性。实验表明重组DAZ3具有体外RNA结合活性，为后续功能研究奠定基础。
2. **文献名称**：DAZ3 Interaction with Germ Cell-Specific RNA Targets Revealed by Recombinant Protein Pull-Down Assays
**作者**：Sasaki, M., & Kato, S.
**摘要**：通过重组DAZ3蛋白的体外Pull-Down实验，鉴定了其在睾丸生殖细胞中结合的特定RNA序列(如PRM1、TNP1)，提示DAZ3在精子发生中可能调控mRNA的稳定性与翻译。
3. **文献名称**：Functional Analysis of DAZ3 in Spermatogenesis Using Recombinant Protein Overexpression Models
**作者**：Zhang, R., et al.
**摘要**：在哺乳动物细胞中过表达重组DAZ3蛋白，发现其可促进生殖细胞系的增殖并抑制凋亡。研究揭示了DAZ3通过MAPK信号通路调控生殖细胞发育的潜在机制。
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**备注**：以上文献为示例性质，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。DAZ3相关研究相对较少，可扩展至DAZ基因家族或生殖细胞蛋白重组表达领域以获取更多信息。
Recombinant human DAZ3 protein is derived from the *DAZ* (Deleted in Azoospermia) gene family, located on the Y chromosome within the AZFc (Azoospermia Factor c) region. This gene family plays a critical role in male germ cell development and spermatogenesis. DAZ3. one of several DAZ paralogs, encodes an RNA-binding protein containing a conserved RNA recognition motif (RRM) and a DAZ repeat domain, facilitating interactions with RNA and proteins involved in post-transcriptional regulation. Mutations or deletions in the AZFc region, including DAZ3. are strongly associated with spermatogenic failure, leading to non-obstructive azoospermia or severe oligozoospermia in men.
The recombinant DAZ3 protein is typically produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its molecular functions. Its production enables in vitro exploration of its role in germ cell maturation, RNA metabolism, and translational regulation during spermatogenesis. Researchers use recombinant DAZ3 to investigate interactions with germ cell-specific RNAs, such as those encoding proteins critical for meiosis or sperm differentiation. Additionally, it serves as a tool for developing diagnostic assays or therapeutic strategies targeting male infertility. Studies also focus on its potential involvement in pluripotency and early embryonic development. Understanding DAZ3's mechanisms could advance treatments for infertility and shed light on conserved reproductive pathways. Overall, recombinant DAZ3 protein remains a key resource for decoding molecular disruptions in spermatogenesis and improving assisted reproductive technologies.
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