| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCLRE1B |
| Uniprot No | Q9H816 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-532 |
| 氨基酸序列 | MNGVLIPHTP IAVDFWSLRR AGTARLFFLS HMHSDHTVGL SSTWARPLYC SPITAHLLHR HLQVSKQWIQ ALEVGESHVL PLDEIGQETM TVTLLDANHC PGSVMFLFEG YFGTILYTGD FRYTPSMLKE PALTLGKQIH TLYLDNTNCN PALVLPSRQE AAHQIVQLIR KHPQHNIKIG LYSLGKESLL EQLALEFQTW VVLSPRRLEL VQLLGLADVF TVEEKAGRIH AVDHMEICHS NMLRWNQTHP TIAILPTSRK IHSSHPDIHV IPYSDHSSYS ELRAFVAALK PCQVVPIVSR RPCGGFQDSL SPRISVPLIP DSVQQYMSSS SRKPSLLWLL ERRLKRPRTQ GVVFESPEES ADQSQADRDS KKAKKEKLSP WPADLEKQPS HHPLRIKKQL FPDLYSKEWN KAVPFCESQK RVTMLTAPLG FSVHLRSTDE EFISQKTREE IGLGSPLVPM GDDDGGPEAT GNQSAWMGHG SPLSHSSKGT PLLATEFRGL ALKYLLTPVN FFQAGYSSRR FDQQVEKYHK PC |
| 分子量 | 60 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人DCLRE1B蛋白的虚拟参考文献示例，基于其已知生物学功能的合理推测：
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1. **文献名称**: *Structural Insights into the DNA Repair Mechanism of Human DCLRE1B*
**作者**: Chen et al. (2021)
**摘要**: 本研究通过X射线晶体学解析了重组人DCLRE1B蛋白的催化核心结构，揭示了其核酸酶活性位点及与DNA末端结合的分子机制，阐明其在非同源末端连接(NHEJ)修复中的作用。
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2. **文献名称**: *Functional Interaction of DCLRE1B with Telomeric Proteins in Genome Stability*
**作者**: Kimura et al. (2019)
**摘要**: 利用重组DCLRE1B蛋白进行体外结合实验，发现其与端粒保护蛋白POT1存在直接相互作用，提示该蛋白在端粒维护和复制压力应答中的双重功能。
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3. **文献名称**: *CRISPR Screening Identifies DCLRE1B as a Sensitizer to PARP Inhibitors in BRCA-Deficient Cancers*
**作者**: Zhang et al. (2022)
**摘要**: 通过CRISPR功能筛选结合重组蛋白活性验证，证明DCLRE1B缺陷可增强BRCA突变癌细胞对PARP抑制剂的敏感性，为其作为联合治疗靶点提供依据。
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注：上述内容为模拟文献，实际引用时请以真实发表的学术论文为准。建议通过PubMed或Web of Science检索关键词"DCLRE1B"或"SNM1B"获取真实文献。
Recombinant human DCLRE1B protein, also known as SNM1B or Apollo, is a DNA repair enzyme encoded by the DCLRE1B gene. It belongs to the SNM1/PSO2 protein family, characterized by a conserved β-CASP metallo-β-lactamase domain that confers nuclease activity. DCLRE1B plays critical roles in maintaining genomic stability, particularly in the repair of DNA interstrand crosslinks (ICLs) and the resolution of stalled replication forks. It is involved in the Fanconi anemia (FA) pathway and interacts with key DNA repair proteins like FANCI-FANCD2 complex.
Studies suggest DCLRE1B contributes to telomere maintenance by processing dysfunctional telomeres and regulating homologous recombination. Its exonuclease activity facilitates the removal of damaged DNA segments, enabling error-free repair. Mutations or dysregulation of DCLRE1B are linked to increased genomic instability, cancer susceptibility, and chemotherapy resistance. Recombinant DCLRE1B is produced using expression systems like E. coli or mammalian cells, allowing functional studies of its enzymatic properties, protein interactions, and role in DNA damage responses. This recombinant protein is vital for investigating molecular mechanisms of genome integrity and developing therapies targeting DNA repair pathways in diseases like cancer.
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